TAILIEUCHUNG - Isolation, morphological and cultural characterization of azospirillum isolated from rhizospheric soils of various non-leguminous crops of ranchi having acidic pH

Azospirillum is one of the versatile non-symbiotic, free living diazotrophic bacteria which appears to have a world-wide distribution and occurs in large number in the rhizosphere soil of a variety of grasses and cereals. The present study was carried out during Rabi and Kharif 2016-17 in the Department of Soil Science and Agricultural Chemistry, Birsa Agricultural University, Ranchi, Jharkhand. Efforts were made to screen out the presence of Azospirillum in rhizosphere of various non-leguminous crops and to characterize the isolates on the basis of morphological and cultural behaviours. | Isolation morphological and cultural characterization of azospirillum isolated from rhizospheric soils of various non-leguminous crops of ranchi having acidic pH 2018 7 8 329-338 International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 7 Number 08 2018 Journal homepage http Original Research Article https Isolation Morphological and Cultural Characterization of Azospirillum Isolated from Rhizospheric Soils of Various Non-Leguminous Crops of Ranchi Having Acidic pH R. Narayan . Gupta and . Shahi Department of Soil Science and Agricultural Chemistry Birsa Agricultural University Ranchi Jharkhand India Corresponding author ABSTRACT Azospirillum is one of the versatile non-symbiotic free living diazotrophic bacteria which appears to have a world-wide distribution and occurs in large number in the rhizosphere soil of a variety of grasses and cereals. The present study was carried out during Rabi and Keywords Kharif 2016-17 in the Department of Soil Science and Agricultural Chemistry Birsa Azospirillum Agricultural University Ranchi Jharkhand. Efforts were made to screen out the presence Rhizosphere of Azospirillum in rhizosphere of various non-leguminous crops and to characterize the Diazotrophic bacteria Isolation isolates on the basis of morphological and cultural behaviours. On the basis of pH range Characterisation 54 rhizospheric soil samples were tentatively selected out of 100 samples for Microcyst Capsule investigation. From the study conducted presence of Azospirillum in rhizosphere of acidic Article Info pH was confirmed. Morphological characterization revealed that Azospirillum isolated from rhizosphere of various crops were gram negative and vibroid in shape. Cells were Accepted encapsulated . were having capsules around them and formed microcyst in aged culture. 04 July 2018 Cultural characterisation revealed that colonies .

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