TAILIEUCHUNG - Phylogenetic analysis of 28S rRNA gene of indigenous xiphinema pachydermum

An attempt has been made to determine the phylogenetic relationship to trace out the evolutionary pattern of the test sequence of Xiphinema pachydermum partial 28S rRNA gene and to find out relationship of the same with other selected sequences of NCBI by constructing phylogenetic trees. Nucleotide sequence of 28S ribosomal RNA gene of Xiphinema pachydermum from Solan, Himachal Pradesh () showed maximum homology of 93% with Xiphinema inaequale isolate HP 28S large subunit ribosomal RNA gene, partial sequence () from Baijnath, Himachal Pradesh. The pairwise similarity score of 38 nucleotide sequences with test sequence elucidated 90-93% sequence identity with other sequences from India. Sequences from South Africa showed 91% identity with the test sequence while sequences from Brazil, Czech Republic, Japan, China and Slovakia showed 90% similarity. USA, Italy, Portugal, Spain, and Canada depicted 89% sequence homology with the Solan isolate. Multiple sequence alignment of 38 selected sequences of Xiphinema species having similarity score between 89 to 93 percent was performed using Clustal Omega and its output was used in Phylip for constructing phylograms. The test sequence, showed maximum closeness with another Indian isolate from Himachal Pradesh, (Xiphinema inaequale) at significant bootstrap values of 100 both by Neighbor Joining and Maximum Parsimony methods respectively, which are further in close proximity to cluster of sequences containing another isolate from Himachal Pradesh, (Xiphinema lambertii isolate XL 28S large subunit ribosomal RNA gene, partial sequence). While close proximity to the sequences from Czech Republic, Slovakia, and Italy, however having low bootstrap value may depict that insect might have migrated from Central Europe to India or vice versa. | Phylogenetic analysis of 28S rRNA gene of indigenous xiphinema pachydermum

• Sinh học
• Phylogenetic analysis
• 28S rRNA gene
• Indigenous xiphinema pachydermum
• Phylogenetic trees
• Xiphinema pachydermum
• Mycoplasma gallisepticum
• Molecular and phylogenetic analysis
• Sequencing and phylogenetic analysis
• Inv E gene
• Inv E gene of salmonella typhimurium
• Salmonella typhimurium
• Listeria monocytogenes
• Labeo catla (Catla fish)
• Dasyatis pastinaca (Stingrays fish)
• Genus Cyclotrichium
• Phylogenetic evaluation of Cyclotrichium
• Morphological revision
• Cytogenetic properties
• Molecular identification
• Phylogenetic tree
• PCR amplification
• Phylogenetic diversity analysis
• Ralstonia Solanacearum
• Pseudomonas Fluorescens
• Strain specific PCR
• Glycoprotein based phylogenetic analysis
• Rabies virus isolates
• Rabies virus in canids
• Glycoprotein sequence based phylogenetic
• BMC Plant Biology
• HMW GS silencing
• Drought stress
• Molecular cloning
• BiP genes
• Triticum aestivum L
• Human transcription
• Phylogenetic relationship
• Few selected human transcription
• Factors sequence analysis
• Computational methods
• BMC Veterinary Research
• Bovine ephemeral fever virus
• Molecular characterization
• Nasal discharge
• Porcine circovirus 3
• Open reading frames
• Viral capsid
• Hepatitis E
• Zoonotic disease
• Pig blood
• Molecular detection
• Lumpy skin disease
• Infectious viral disease
• Roe deer
• Next generation sequencing
• Taxonomic classification
• Molecular relationship
• Foot and mouth disease virus serotype
• Indian vaccine strain
• World by phylogenetic analysis
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Tripleurospermum eskilensis
• Morphological characters
• Molecular phylogenetic analysis
• Gossypium sp
• SSR marker
• Cluster analysis
• Genetic diversity
• Phylogenetic relationships
• Potential cotton
• Pseudoroegneria species
• Chloroplast trnL F sequences
• Chloroplast DNA
• ITS sequences
• MatK sequences
• Molecular phylogenetic
• Onobrychis species
• Onobrychis Mill
• Internal transcribed spacers
• Genus Aeschynomene
• Phylogenetic framework
• Nod dependent
• Nod independent symbioses
• Genetic relationship
• Genetic distance
• Microsatellite markers
• Zebu cattle
• BMC Bioinformatics
• Genome evolution
• Regulatory evolution
• Genomic algorithms
• Ancestral reconstruction
• Salmonella enterica
• 16S RNA
• Biochemical tests
• Salmonella enterica based on 16S RNA
• Taxonomic identification
• Phylogenetic position
• Archaeal organisms
• 16S DNA sequences
• Plant Biology
• Chloroplast genome
• Molecular markers
• Species identification