TAILIEUCHUNG - Detection of various β-lactamases and their co-production by novel disc placement method among gram negative bacilli isolates from patients with spontaneous bacterial peritonitis

Gram-negative bacilli (GNB) are most frequently isolated bacteria from ascitic fluid aspirate of patients with Spontaneous bacterial peritonitis (SBP). Aetiology of SBP has been changing in recent years with increased involvement of multidrug resistant (MDR) bacteria as a result of production of various β-lactamases. The present study was done for detection of different β-lactamases and their co-existence by noveldisc placement method among GNB isolates from patients with SBP. Disc panel containing ceftazidime (CA), ceftazidime/tazobactam (CA/TZ), cefepime (CFP), ceftazidime/ tazobactam/ cloxacillin (CA/TZ/CLOX), imipenem (IMP) and Mercaptopropionic acid (MPA) was used. Pureextended spectrum β-lactamase (ESBL) production was found in 18 of 42 isolates (). Maximum ESBL positive isolates were found in (13 out of 28 isolates).Pure AmpC production was found in 6 of 42 isolates ().Metallo-βlactamases (MBL) production was found in only 2 of 42 isolates (). Co-production of ESBL+ AmpC was seen in 3 of 42 isolates (). All ESBL positive isolates showed multidrug resistance pattern. Detection of β-lactamase producing isolates will provide knowledge regarding resistance pattern of bacterial strains in a particular geographical area. This will further help promote appropriate and judicious use of antibiotics. | Detection of various β-lactamases and their co-production by novel disc placement method among gram negative bacilli isolates from patients with spontaneous bacterial peritonitis

• Y khoa - Dược
• β lactamases
• Novel disc panel
• Co production by novel disc placement method
• Gram negative bacilli isolates
• Spontaneous bacterial peritonitis
• Summary of the thesis
• The clinical of spontaneous bacterial peritonitis
• Laboratorial features of spontaneous bacterial peritonitis
• Treatment of spontaneous bacterial peritonitis in cirrhosis
• Peritonitis infection spontaneously
• Liver cirrhosis
• Prognostic marker
• Diagnostic value of ascitic fluid lactoferrin
• Ascitic fluid
• Ascitic fluid culture
• Gram stain
• Tertiary care hospital
• Patients with spontaneous bacterial peritonitis
• Cirrhosis MELD
• Predictors of in hospital mortality
• Cirrhotic patients
• Abdominal organ transplant patients
• Hepatic hydrothorax
• Refractory ascites
• Hepatic encephalopathy
• Upper gastrointestinal bleeding
• Conventional method
• Molecular biology
• Patients with ascites
• Sequencing in the diagnosis
• Role of culture