TAILIEUCHUNG - Evaluation of sterilant effect on in-vitro culture establishment in sugarcane variety Co 0118

In-vitro propagation is widely used as reliable alternative for true-to-type and quality seed production in sugarcane. The main obstacle under in-vitro propagation is growth of contaminants, resulting in failure of culture establishment that can be minimized by effective surface sterilization method. The sterilization process varied species to species and explants type thus, present study was designed to evaluate the effects of various sterilants on in-vitro culture establishment of sugarcane variety Co 0118 through meristem shoot tip. The explants were collected and treated with different concentrations of sterilants viz., BavistinTM (), HgCl2 (), NaOCl (2-10%) and EtOH (60- 100%). The treated explants were inoculated on semi solid MS media supplemented with mg/L each of BAP and Kn. The results obtained under study showed significant differences in their activity. The optimum culture survival percent and lower contamination with reduced necrosis was to be found when explants were treated with the combination of Bavistin for 10 min followed by HgCl2 for 5 min, 6% NaOCl for 10 min and 70% EtOH for 1 min. This sterilization protocol will be contributive in efficient surface sterilization for in-vitro propagation of sugarcane though meristem shoot tip. | Evaluation of sterilant effect on in-vitro culture establishment in sugarcane variety Co 0118

• Sinh học
• Meristem shoot
• Sugarcane variety Co 0118
• In vitro culture establishment
• Evaluation of sterilant
• Production in sugarcane
• BMC Plant Biology
• Shoot meristem
• Plant embryo
• Angiosperm evolution
• Complex functional redundancy
• Arabidopsis thaliana embryos
• Plant Biology
• Chemically induced mutation
• Lateral shoot inhibition
• RNA seq
• Tobacco farmers
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Aerial stem
• Biological hardening
• Direct regeneration
• Primary thickening meristem
• Trichoderma harzianum
• Head formation
• High temperatures
• Genomic analysis
• Brassica oleracea var italica
• BMC Genomics
• Shoot apical meristem
• Argonaute proteins
• Directing sRNAs
• Spikelet meristems
• Regulating spikelet meristems
• Knowledge of farmers
• Production technology
• Production technology of sugarcane
• Sugarcane in SRI
• Sugar production
• Price risk
• Risk analysis in sugarcane production
• Sugarcane production
• Evidences from Uttar Pradesh
• Maharashtra states of India
• Facing several risks
• Comparative analysis in cost
• Returns of sugarcane production
• Exhibited inverse relationship
• Type of ownership of land
• Biodiesel Feedstock Production
• Freshwater Microalgae Grown
• Sugarcane Juice Hydrolysate
• Microalgae derived biodiesel
• The heterotrophic growth
• Sugarcane thick juice
• Dynamics of area
• Productivity and trade
• Sugarcane in India
• Major sugarcane producing
• Cost of cultivation
• Net return
• Benefit cost ratio
• An economic analysis
• Production of sugarcane
• Methods of irrigation
• Gross return
• Input output ratio
• Sugarcane production in baghpat district
• Diazotrophic bacteria
• Influence of diazotrophic bacteria
• Diazotrophic bacteria on growth
• Biomass production
• Sugarcane invitro
• Nonlinear regression models
• Stanadard error etc
• Growth in area
• Statistical model to analyse
• Cost and revenue
• Input use efficiency
• Constraints analysis
• Sugarcane cultivation
• Economics analysis of production
• Lignocellulosic biomass
• Sugarcane bagasse
• Ultrasound assisted alkaline pretreatment
• Hydrolytic enzymes
• Bacillus thuringiensis
• Sugarcane juice
• Biopolymer polyhydroxybutyrate
• Bacillus spp
• Nitrogen deficient
• Osmotic stress
• Sugarcane (Saccharum officinarum L
• Antioxidant enzymes respond
• Expansion of production
• Crop plants
• Glycine beaten
• Salinity stress
• Osmoprotectants securing sugarcane
• Elevated levels
• Production and salinity stress
• Growth performance
• Growth and instability analysis
• Production and productivity trend
• Economic perspectives
• Sugarcane cultivation in Bihar
• Rhizospheric mycoflora
• Plant growth promotion
• Plant growth promotion of sugarcane
• Oxidase production
• Aureobasidium pullulans
• Novel media optimized
• Production of pullulan
• Flask type fermentation system
• Milky mushroom
• Sugarcane baggase
• Niger stick
• Production of milky mushroom
• Evaluation of different substrates
• Nutrient availability
• IAA production
• ACC deaminase
• Saccharum officinarum L
• Nonlinear models
• Root mean square error
• Mean absolute error
• Durbin Watson statistic
• Levenberg Marquardt technique
• Shapiro Wilks statistic
• Cost economics
• Lactation trial
• Murrah buffaloes
• Milk yield
• Milk quality
• Sugar cane by products
• Pleurotus ostreatus
• Pleurotus sajor caju
• Solid state fermentation
• Cellulosic components
• Microbial enzyme