TAILIEUCHUNG - Isolation and optimization of the growth conditions of thermophilic microorganism from hot springs

The aim of this study was to isolate and optimize the growth conditions of thermophilic microorganism from hot springs. The isolation was conducted by using the mineral salt basal medium supplemented with yeast extract at 500C. Totally, 33 isolates of thermophilic microorganism were isolated from hot springs at Truong Xuan (Khanh Hoa province) and Binh Chau (Ba Ria - Vung Tau province). The effects of temperature (45 - 800C), pH (pH 6 - 9) and carbon sources (malate, pyruvate, acetate, glucose, fructose, or carbon dioxide) on the growth of isolates were examined. In addition, the isolate morphology was also investigated by Gram and spore staining. The isolated thermophilic microorganism showed the diversity in colony morphology and color appearance. Most of them were rod shaped, spore-forming and most grew well at 500C and pH 7. The highest growth of all isolates was observed under malate, glucose, or fructose, as an organic carbon source and unable to use carbon dioxide. Six out of 33 thermophilic microorganism isolates (namely BM7, BS5, NS1, NS3, NS4, and NW6) grew rapidly under high temperatures from 50 - 550C and their morphology characteristics showed high similarity to Bacillus sp. The study evidenced the polymorphic diversity of thermophiles in the geothermal hot spring ecosystems. | Isolation and optimization of the growth conditions of thermophilic microorganism from hot springs

• Sinh học
• Isolation and optimization
• Growth conditions
• Thermophilic microorganism
• Hot springs
• Geothermal hot spring ecosystems
• Optimization protocol
• DNA isolation
• PCR in Muskmelon
• RAPD marker
• Muskmelon (Cucumis melo L )
• Isolation of the growth conditions
• Optimization of the growth conditions
• Thermophilic microorganism from hot springs
• Mophilic microorganism isolates
• Lactic acid bacteria
• Bacteriocin production
• Isolation of probiotic bacteria
• Probiotic bacteria
• Optimization of physical
• Nutrition parameters
• Biochemical analysis
• Antidesma bunius
• Wild fruit
• Nutritional profiling
• Efficient nucleic acid isolation protocol
• Recalcitrant tissue
• Agro industrial
• Pineapple waste
• Pectin from pineapple wastes
• Process optimization
• Various pineapple waste biomass
• Media optimization
• Scytilidium lignicola
• Submerged fermentation
• Scytalidium lignicola pesante
• Optimization for laccase production
• Pseudomonas sp
• Soil rhizosphere and marine water
• Optimization of siderophores
• Siderophores (Iron Chilators)
• Pseudomonas fluorescence NCIM 5096
• Wheat bran
• Agro industrial wastes
• Optimization of alkaline amylase production
• Bacillus cereus
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo thú y
• bệnh ở động vật
• nghiên cứu bệnh thú ý
• Oryza sativa
• Endophytic fungi
• Condition Optimization
• GA3 in submerged fermentation
• Biocontrol agent
• Cultural conditions of submerged fermentation
• Partial purification
• Alkaline chitinase enzyme
• Fusarium sp
• Phytic acid
• Pseudomonas aeruginosa
• Aspergillus niger
• Poultry faeces
• Aspergillus niger isolated