TAILIEUCHUNG - Effect of heat stress and amelioration by antioxidants on expression profile of pro- and anti-apoptotic genes in in vitro matured bovine oocytes

Heat stress often leads to apoptosis of oocytes through generation of free radicals. The use of antioxidants has been found to mitigate the harmful effects of these free radicals and probably apoptosis itself. The present study was conducted to evaluate the effect of heat stress on expression profile of genes related to apoptosis (pro-apoptotic Bad and Bax; and anti-apoptotic Bcl-2) during oocyte maturation and the ameliorating effects of select antioxidants- viz. melatonin and zinc. In the experiment, bovine oocytes were divided into 4 groups and Group II, III, IV was matured under heat-stress at 41°C. Moreover, group III and IV were supplemented with antioxidant melatonin and zinc respectively, incorporated in the oocyte maturation medium (OMM), while Group II served as antioxidant control and was matured with OMM alone. Group I served as control and was matured without heat-stress (°C) and antioxidant supplementation. After maturation, the total RNA was isolated for Bcl-2, Bax and Bad expression. It was found that there was up regulation of Bad and Bcl-2 gene expression during induced heat-stress without any supplementation (Group-II). Bax was down regulated in all groups, while Bad was down-regulated in melatonin and zinc supplemented groups. It is speculated that supplementation with zinc probably induced early maturation changes in the oocyte and induced an early meiotic arrest, which was associated with a sharp decline in all apoptosis modulator transcripts. It sis concluded that by detoxifying ROS, antioxidants may therefore subsequently reverse the ROS-induced decline in Bcl-2 and prevent apoptosis. | Effect of heat stress and amelioration by antioxidants on expression profile of pro- and anti-apoptotic genes in in vitro matured bovine oocytes

• Sinh học
• Heat stress
• Gene expression
• In vitro matured bovine oocytes
• Pro and anti apoptotic genes
• Expression profile
• Amelioration by antioxidants
• BMC Genomics
• Populus qiongdaoensis
• Heat shock protein
• Heat stress tolerance
• Small heat shock protein
• Heat susceptibility index
• Thermal stress tolerant
• Bread wheat
• Heat stress tolerance for yield
• Contributing attributes
• Farm workers
• Heat stress on farm workers
• Policy making strategies implementation
• Heat stress on farmers
• Combined stress
• Canopy temperature
• Variation in NDVI
• Individual heat
• Combined heat drought stresses in wheat
• Thermal stress
• Tasar silkworm
• Tropical tasar silkworm
• Heat shock
• Heat illness
• Organ damage
• Risk assessment strategies
• Heat transfer type
• Residual stress distribution
• Welding residual stress
• Finite element method
• Heat transfer method
• Heat stress on poultry production
• Poultry production
• Their managemental approaches
• Relative humidity
• Path analysis
• Grain yield and heat stress
• Maize (Zea mays L
• Grain yield
• Character association and path analysis
• Grain weight
• Zinc nutrition
• Mitigating late sowing induced heat stress
• Wheat crop
• Vitamin C
• Corn oil
• Broiler performance under heat stress
• Corn oil supplementation
• Dietary vitamin C
• Triticum aestivum L
• Proteomic analysis in wheat
• Effect of heat stress on flag leaf
• Heat stress on flag leaf
• Crop improvement programme
• Flag leaf
• Booting and heat stress
• Wheat (Triticum aestivum L )
• Grown under heat stress
• Boron accumulation in flag leaf
• Physiological analysis
• Heat stress on wheat genotypes
• Wheat genotypes
• Plant material comprises
• Ascorbic acid
• Commercial broilers
• Heat stress in commercial broilers
• Biochemical parameters
• Ascorbic acid on biochemical parameters
• Plant Biology
• Creeping bentgrass
• Abiotic stress
• Especially heat stress
• Tamarix hispida
• BMC Veterinary Research
• Dairy cattle
• Vascular endothelial growth factor
• Heat stressed cattle
• Public health
• Agricultural workers
• Core body temperature
• Heat related illness
• Heat strain
• Heat tolerance
• Temperature stress
• Heat tolerance in wheat
• Date of transplanting
• Enzymatic antioxidants
• Nonenzymatic antioxidants
• Sensitivity to heat stress
• Ipomoea aquatica
• Long term heat stress
• RNA seq
• Starch and sucrose metabolism
• Pearl millet
• Pacbio sequencing
• Illumina sequencing
• Drought stress
• BMC Plant Biology
• Bax inhibitor 1
• Heat responsive genes
• Heat stress response
• Adrenal glands
• Transcriptional regulation
• Cleistogenes songorica
• RNA sequencing
• Conserved heat responsive genes
• Co expression
• Regulatory network