TAILIEUCHUNG - In vitro propagation of finlayson’s cymbidium orchid

Finlayson’s Cymbidium is a native species in Vietnam, and this is one of the orchids favorite for many orchidists. In order to enrich this species, the propagation was conducted in vitro. The experiments were carried out to germinate protocorm of orchid seeds, multiply shoots and develop the complete plant formation. | Hue University Journal of Science Agriculture and Rural Development ISSN 2588-1191 Vol. 126 No. 3E 2017 DOI IN VITRO PROPAGATION OF FINLAYSON S CYMBIDIUM ORCHID Bui Quoc Minh Phan Thi Phuong Nhi HU - University of Agriculture and Forestry 102 Phung Hung St. Hue Vietnam Abstract Finlayson s Cymbidium is a native species in Vietnam and this is one of the orchids favorite for many orchidists. In order to enrich this species the propagation was conducted in vitro. The experiments were carried out to germinate protocorm of orchid seeds multiply shoots and develop the complete plant formation. The experiments were organized in a completely randomized design with three replications for each test medium. The results showed that the MS medium supplemented with 20 g L saccharose 6 g L agar 1 g L activated charcoal 10 coconut water and 1 mg L 6-benzyladenine was the optimal medium for protocorm shooting. In the shoot multiplication stage the MS medium supplemented with 1 g L activated charcoal 20 g L saccharose 6 g L agar and 2 mg L 6-benzyladenine was the most suitable. For rooting and developing plant formation the suitable medium was MS supplemented with 20 g L saccharose 6 g L agar 1 g L activated charcoal and mg L 1-naphthaleneacetic acid. The highest roots and leaves per plant were found. The plant height was cm after the 6-week culture. Keywords Cymbidium in vitro shoot multiplication 1 Introduction Finlayson s Cymbidium Cymbidium finlaysonianum Wall. ex Lindl. 1833 called Hoang Kiem Lan in Vietnamese is one of the orchids favorite to many orchidists. This is a native species of Vietnamese orchid belonging to the Orchidaceae family Cymbidium genus. This genus consists of approximately 50 varieties in the world. There are 31 varieties in Vietnam. The genus of Cymbidium grows on trees rocky cliffs and in limestone soil. This genus has long narrow leaves cone-shaped pseudobulbs that are encased in the leaf bases.

• Nông nghiệp
• Finlayson’s Cymbidium
• In vitro propagation
• Native species in Vietnam
• Shoot multiplication
• Dendrobium anosmum
• Rhynchostylis gigantea
• Tạp chí Khoa học Công nghệ Nông nghiệp
• Bài viết về nông nghiệp
• Lan Đai Châu
• Kỹ thuật chăm sóc lan Đai Châu
• Vi nhân giống lan Đai châu
• Mô sẹo phôi hóa
• Rhynchostylis gigantea L
• Tái sinh chồi
• Tế bào phôi
• chất điều hòa sinh trưởng
• nhân giống lan ngoch
• lan ngọc điểm đỏ
• tài liệu nông nghiệp
• nghiên cứu nông nghiệp
• cụm chồi lan
• Biến đổi sinh lí cây phong lan Đai châu
• Cây phong lan Đai châu
• Hóa sinh của cây phong lan Đai châu
• Nguồn gốc in vitro
• Cây phong lan Đai châu ở nhà lưới