TAILIEUCHUNG - Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

Bupleurum latissimum Nakai is a critically important endangered plant belonging to the family Apiaceae. Seed germination was promoted by soaking in the dormancy breaker gibberellic acid (GA3) (50% germination as compared with 4% of the control). An optimal temperature for germination of seed previously soaked in GA3 solution was determined by incubation at various temperatures. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 732-739 © TÜBİTAK doi: Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis Kee Hwa BAE* National Institute of Biological Resources, Seo-gu, Incheon, Republic of Korea Received: Accepted/Published Online: Printed: Abstract: Bupleurum latissimum Nakai is a critically important endangered plant belonging to the family Apiaceae. Seed germination was promoted by soaking in the dormancy breaker gibberellic acid (GA3) (50% germination as compared with 4% of the control). An optimal temperature for germination of seed previously soaked in GA3 solution was determined by incubation at various temperatures. Seed germination of was observed at 25 °C. These results indicate that the seeds of B. latissimum are difficult to germinate, even when treated with GA3. The greatest callus induction () was observed in root explants of seedlings grown on MS medium containing a specific concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) ( mg L–1) and N6-benzyladenine (BA) ( mg L–1). Induction of somatic embryo was observed in of the root segment cultured on MS medium containing mg L–1 2,4-D alone. The highest shoot induction rate was obtained in MS medium containing 30 g L–1 sucrose (number of shoots, ; average length of shoot, cm). After acclimation in artificial soil (1:1:1 mixture of peat moss, perlite, and sand) and transfer to the greenhouse, 98% of plantlets survived over 2 months. This in vitro propagation protocol will be very useful for the conservation of this critically endangered plant. Key words: Bupleurum latissimum Nakai, callus, endangered, endemic, GA3, regeneration 1. Introduction The genus Bupleurum belongs to the family Apiaceae and comprises approximately 200 species, mainly distributed throughout Eurasia. Most

• Sinh học
• Bupleurum latissimum Nakai
• Seed germination
• In vitro propagation
• Breaker gibberellic acid
• Critically endangered plant
• Aconitum heterophyllum
• Critically endangered
• Anther dehiscence
• Pollen germination
• Indole 3 butyric acid
• Indole 3 acetic acid
• Erodium somanum
• Reproductive biology
• Endangered endemic species
• Dioecious plant
• Conservation needs
• Plant Biology
• Plastid genome
• Plastid gene loss
• Photosynthetic function
• Saniculiphyllum guangxiense
• Nuclear paralogs exist
• Journal of Botany
• Ferula sinkiangensis
• Genetic assessment
• Small population size
• Medicinal plant
• Plant’s natural habitats
• Anthemis xylopoda
• Endemic plant
• Adventitious shoot regeneration
• In vitro flowering
• Indole3 acetic acid
• Arnebia euchroma
• Microbial diversity
• Shannon Wiener index
• Microbial load
• Medicinal plants