TAILIEUCHUNG - Changes in gene expression in SIRT3 knockout liver cells

The sirtuin (SIRT) gene family is reported to regulate critical intracellular processes from aging to cellular metabolism and repair. SIRT3 knockout (SIRT3-/-) mice develop receptor positive mammary tumors starting at 13 months and SIRT3 expression is decreased in human breast cancer samples as well as several other diseases. | Turkish Journal of Biology Turk J Biol (2015) 39: 380-387 © TÜBİTAK doi: Research Article Changes in gene expression in SIRT3 knockout liver cells 1 1 2 1 2 1 1,2, Randa TAO , Jamie LECLERC , Kübra YILDIZ , Seong-Hoon PARK , Barbara JUNG , David GIUS , Özkan ÖZDEN * 1 Department of Radiation Oncology, Robert Lurie Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA 2 Department of Medicine, Division of Gastroenterology and Hepatology, University of Illinois at Chicago, Chicago, IL, USA Received: Accepted/Published Online: Printed: Abstract: The sirtuin (SIRT) gene family is reported to regulate critical intracellular processes from aging to cellular metabolism and repair. SIRT3 knockout (SIRT3-/-) mice develop receptor positive mammary tumors starting at 13 months and SIRT3 expression is decreased in human breast cancer samples as well as several other diseases. It is established that carcinogenesis results from the accumulation of multiple aberrant genetic events including the activation of oncogenes and inactivation of tumor-suppressor genes. To determine the potential early genomic events that may play a role in the tumor-permissive phenotype observed in SIRT3-/- cells, we compared gene expression profile in SIRT3-/- and wild-type (SIRT3+/+) mouse livers. Differences between the expression profiles of genes important in the p53 and apoptosis pathway and signal transduction pathways, as well as genes involved with insulin and cholesterol metabolism, were determined. These results demonstrate that the expression of several oncogenes including Cdkn1, Myc, and Nos2 are increased. In contrast, several genes shown to be downregulated in human breast cancer including Btg2, Egr-1, Fos, Jun, Gadd4, and Wnt1 had decreased expression. The current work demonstrates that the loss of function of SIRT3 results in a cellular .

• Sinh học
• Gene expression
• SIRT3 knockout liver cells
• Human breast cancer
• Reactive oxygen species
• ADP ribosyltransferases
• BMC Genomics
• Gene expression profiling
• Light response
• Basal level gene expression
• Genome duplication
• Physiological regulators
• Recombinant therapeutic protein
• Transient gene expression
• Different expression systems
• Recent advances
• Transient gene expression protocol
• Cell lines used
• BMC Bioinformatics
• Single cell RNA seq
• Differential gene expression
• Stochastic gene expression
• Transcriptional bursting model
• BMC Plant Biology
• Tomato expression database
• NGS data analysis
• Expression Atlas
• Gene expression comparison
• Gene expression data
• Gene network
• Simulate gene
• Stochastic simulation algorithm
• Escherichia coli M15
• Burkholderia cepacia in escherichia coli
• Catechol 1
• 2 dioxygenase gene
• Improved expression of catechol 1
• Genus Citrus
• Gene function
• Co expression networks
• Genome wide scale
• Brassica spp
• Digital gene expression
• Brassica includes
• Diploid progenitors
• Displaying complex
• The inflammatory gene expression
• Horse oil in DNCB treated mice skin
• The inflammatory gene expression by horse oil
• DNCB treated mice skin
• Including severe erythema
• Intron evolution
• Transcript evolution
• RNA processing
• Coding sequence
• RNA Seq
• Expression profile
• Gene networks
• Gene candidates
• Querying genes
• Gene expression studies
• Statistical test
• Fold change criterion
• Gene expression atlas
• Cucurbita pepo
• Novel genes
• Implementing gene expression programming
• The parallel environment for big datasets classication
• Big datasets classication
• Gene expression programming
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• BMC Veterinary Research
• Antioxidant capacity
• Laying hens
• Immunoregulatory function
• Change point
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• Time series gene expression data
• Dynamic information
• Model interpretation
• PolyA selection
• Gene level expression estimates
• Cloning and expression of pigc gene
• Pigc gene in escherichia coli
• Escherichia coli
• Pigc gene
• Producing large scale prodigiosin
• Set level classification
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• Biologically interpretable gene sets
• Gene ontology
• Supervised learning
• RNA sequencing
• Functional genomics
• ChIP seq
• ChIP chip
• Gene expression omnibus
• Data integration
• Gene regulation
• Distributed representation
• Gene co expression
• Gene gene interaction
• Existing functional description
• Gene embedding
• Biotechnology and food sciences
• Listeria monocytogenes strain BN3
• Acyl homoserine lactone
• Expression of virulence gene
• Gene responsible for biofilm
• Genome Biology
• Interpolated Markov model
• Enhancer to target gene assignment
• Drosophila melanogaster
• Machine learning
• Histochemical assay
• Reporter gene
• Transient expression
• Brinjal leaves through agroinfiltration
• Transient expression of reporter gene