TAILIEUCHUNG - Detection of four apple viruses by ELISA and RT-PCR assays in Turkey

Plant samples were collected from the main apple growing provinces of Turkey in order to evaluate the incidence of 4 important apple virus diseases during spring 2004. Collected leaves and shoots were tested using enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) for Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV) and Apple chlorotic leaf spot virus (ACLSV). | Turk J Agric For 30 (2006) 241-246 © TÜB‹TAK Detection of Four Apple Viruses by ELISA and RT-PCR Assays in Turkey Kadriye ÇA⁄LAYAN1,*, Çi¤dem ULUBAfi SERÇE1, Mona GAZEL1, Wilhelm JELKMANN2 1 Mustafa Kemal University, Agriculture Faculty, Plant Protection Department, 31034 Antakya - TURKEY 2 Federal Biological Research Center for Agriculture and Forestry, Institute for Plant Protection in Fruit Crops, Dossenheim - GERMANY Received: Abstract: Plant samples were collected from the main apple growing provinces of Turkey in order to evaluate the incidence of 4 important apple virus diseases during spring 2004. Collected leaves and shoots were tested using enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) for Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV) and Apple chlorotic leaf spot virus (ACLSV). Since no commercial antiserum is available, Apple stem pitting virus (ASPV) was tested only using RT-PCR and the results were compared. A total of 174 apple samples were collected from varietal collections belonging to governmental and university institutions and also from commercial orchards. Out of 174 plants, 126 were infected by at least one virus disease. The incidence of the 4 viruses in varietal collections was , while it was in commercial orchards. The results obtained from the comparison of ELISA and RT-PCR in this study showed that with the RT-PCR technique more samples were positive for ACLSV, ASGV and ASPV. Mixed infections were also very common in both varietal and commercial orchards. Among the mixed infections, the most common one was ASPV + ACLSV (), followed by ASPV + ASGV (), ACLSV + ASGV () and ASPV + ApMV (). The incidence of the ASPV + ASGV + ACLSV combination was . The number of plants infected with any viruses was higher when tested using RT-PCR comparing to ELISA. This study showed that apple virus diseases, especially on .

• Nông nghiệp
• Enzyme linked immunosorbent assay
• Apple mosaic virus
• Apple chlorotic leaf spot virus
• Apple stem grooving virus
• Apple stem pitting virus
• Indirect enzyme linked immunosorbent assay
• Recombinant microneme 10
• Protein of toxoplasma gondii
• Toxoplasma gondii for diagnostic evaluation
• Murine model infected
• BMC Veterinary Research
• Porcine reproductive
• Respiratory syndrome virus
• M protein
• Synthetic peptide
• Enzyme linked immune electrotransfer blot
• Human cysticercosis
• Tertiary care center
• Seroprevalence of human cysticercosis
• BMC Biotechnology
• Human enteropeptidase light chain
• Protein protein coupling
• Surface supercharging
• Enzyme antibody
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• Kỹ thuật ELISA
• Máy đọc ELISA
• chuẩn đoán bệnh lao
• báo cáo khoa học
• tài liệu về y học
• tình trạng nhiễm bệnh
• kháng nguyên kháng thể
• Citrus tristeza virus
• Western blot
• Biological indexing
• Double antibody sandwich
• Molecular characterization
• Reverse transcription
• Plum pox potyvirus
• Restriction analysis
• Reverse transcription polymerase chain reaction
• Monoclonal antibodies
• Xylella fastidiosa
• Pierce’s disease
• Xylem limited bacterium
• Gram negative bacterium
• Oral health
• Saliva mucins
• Dental caries
• Enzyme linked immunosorbent sandwich assay
• Receiver operating characteristic
• NS1 antigen
• Rapid immuno chromatographic test
• Dengue NS1 antigen
• Microwell ELISA
• BMC Pharmacology and Toxicology
• Bone mineral density
• Perinatal phencyclidine
• Serum spectrophotometrically
• Hericium erinaceus
• Cyathane diterpenoids
• High performance liquid chromatography
• Cyathus helenae
• BMC Chemistry
• Graphene oxide
• Gold nanoparticles
• Atom transfer radical polymer
• BMC Cancer
• Apelinergic system
• Chronic lymphocytic leukaemia
• Enzyme linked immunosorbent assay kits
• Manufacturer’s instructions
• Polymerase chain reaction
• CMV DNA
• Pregnant women
• Detection of cytomegalovirus
• Advanced non small cell lung cancer
• Glasgow prognostic score
• Carcinoembryonic antigen
• Non small cell lung carcinomas
• Receiver operating characteristic curve
• Binding antibody multiplex assay
• Quality control
• LabKey Server platform
• Humoral responses
• Enzyme linked immunosorbent assays
• Infectious bronchitis virus
• Peptide ELISA
• Neutralizing antibody
• Infectious bronchitis
• Monoclonal antibodies specific
• Serum progesterone
• Hybrid cells
• Hybridoma technology
• Devastating psychiatric disorder
• Potential serum biomarkers
• First episode psychosis
• Brain derived neurotrophic factor
• Non structural protein 1
• HEK293T cell
• Secretory expression
• Zika virus