TAILIEUCHUNG - Somatic embryogenesis from immature cotyledons of apomictic and non apomictic seeds in walnut (Juglans regia L.)

Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. | Turk J Agric For 30 (2006) 111-117 © TÜB‹TAK Somatic Embryogenesis from Immature Cotyledons of Apomictic and Non-Apomictic Seeds in Walnut (Juglans regia L.) Bekir fiAN, Hatice DUMANO⁄LU* Ankara University, Faculty of Agriculture, Department of Horticulture, 06110 Ankara - TURKEY Received: Abstract: Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen of the apple cv. ‘Golden Delicious’ (Malus x domestica Borkh.). The best cotyledon stage for somatic embryogenesis was determined in openpollinated seeds of 10 walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after anthesis. As a result of this experiment, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after anthesis. Driver and Kuniyuki -1 -1 -1 walnut (DKW) medium supplemented with 1 mg l 6-benzylaminopurine (BAP), 2 mg l kinetin, mg l indole-3-butyric acid -1 (IBA) and 250 mg l L-glutamine was used in initial cultures. Explants were transferred to DKW medium without growth regulators and L-glutamine in subcultures. The percentage of embryogenic cotyledons that originated from apomictic and non-apomictic seeds ranged from to 25% and the number of embryos per cotyledon ranged from 1 to at the end of the fourth subculture. A repetitively embryogenic embryo line originating from immature cotyledons of apomictic seeds of the Tokat-1 walnut genotype was maintained by secondary embryogenesis. Key Words: Walnut, Juglans regia L., somatic embryogenesis, immature cotyledon, apomictic seed Cevizde (Juglans regia L.) Apomiktik ve Apomiktik Olmayan Tohumlar›n Olgunlaflmam›fl Kotiledonlar›ndan Somatik Embriyogenesis Özet: Baz› ceviz (Juglans regia L.) genotiplerinde apomiktik ve aç›kta tozlanm›fl tohumlar›n olgunlaflmam›fl kotiledonlar›ndan somatik embriyogenesis .

• Nông nghiệp
• Walnut Juglans regia L
• Somatic embryogenesis
• Immature cotyledon
• Apomictic seed
• Walnut genotype
• Saturated salt solutions
• Somatic embryos
• Gibberellic acid
• Walnut somatic
• genotypes
• Economical seed
• Apomictic fruit set
• Turkish walnut genotypes
• Gametophytic apomixis
• BMC Chemistry
• Large scale preparation
• Walnut peptides
• Juglans regia L
• Obtained peptides
• BMC Genomics
• Colletotrichum gloeosporioides
• Long non coding RNAs
• Walnut anthracnose
• Association genetics
• Germplasm collection
• Linkage map
• QTL analysis
• Bearing habit
• Different pre sowing treatments
• Seed germination
• Seedling growth of walnut
• Early germination of walnut seed
• BMC Plant Biology
• Juglans sigillata Dode
• Self pollination
• Juvenile period
• Plant Biology
• Rapid increase
• Walnut explants
• Gene expression assays
• Báo cáo lâm nghiệp hay
• cách trình bày báo cáo
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp
• Neuro fuzzy
• Walnut cultivars
• Color index
• Hue angle
• CR 5 Konica Minolta
• NF Y transcription factor
• Phylogenetic analysis
• Expression profiles
• Transcriptome sequencing
• Fatty acid
• Gene expression
• FAD gene family
• Linoleic acid
• Walnut kernels contain
• Journal of Agriculture and Forestry
• Genetic resources
• Fatty acids
• Germplasm sources
• Tocopherol isomers