TAILIEUCHUNG - Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat
Ischemia/reperfusion (I/R) is one of the main causes of acute renal failure, leading to generation of reactive oxygen species (ROS) and ensuing oxidative stress, which results in unfolded misfolded protein accumulation and its dependent response pathways, generally known as unfolded protein response (UPR), in the endoplasmic reticulum (ER). | Turkish Journal of Biology Turk J Biol (2013) 37: 464-471 © TÜBİTAK doi: Research Article Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat 1 1 2 3 4 Khadijeh HASHEMI , Kazem PARIVAR , Samira FAZLIFAR , Alireza HAGHPARAST , Mehrdad MOHRI , 5 5 2, Naser MARGAN AZGHADI , Mohammadhossein NAZEM SHIRAZI , Amir AFKHAMI-GOLI * 1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 3 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 5 Khorasan Razavi Veterinary Office, Mashhad, Iran Received: Accepted: Published Online: Printed: Abstract: Ischemia/reperfusion (I/R) is one of the main causes of acute renal failure, leading to generation of reactive oxygen species (ROS) and ensuing oxidative stress, which results in unfolded/misfolded protein accumulation and its dependent response pathways, generally known as unfolded protein response (UPR), in the endoplasmic reticulum (ER). We studied the effects of renal I/R on the expression of ER-stress genes, as well as concomitant effects of oral pretreatment with pomegranate (Punica granatum L.) pith and carpellary membrane (PPCM). An in vivo model of rat kidney I/R followed by conventional and real-time RT-PCR was used to evaluate the modulation of GRP78 and XBP1 as central UPR and ER-stress markers. Ischemia followed by reperfusion (60 and 150 min, respectively) resulted in decreased antioxidant properties of plasma (lowered ferric reducing ability of plasma [FRAP] value) and GRP78 transcript levels. Oral administration of PPCM aqueous
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