TAILIEUCHUNG - Molecular detection of antibiotic related genes from Pseudomonas aeruginosa FP6, an antagonist towards Rhizoctonia solani and Colletotrichum gloeosporioides

Despite the importance of antibiosis in biological control, little is known about the genes involved in antifungal activity. Therefore, the present study was aimed at identifying the location of the antagonistic gene(s) in Pseudomonas aeruginosa FP6 towards Rhizoctonia solani and Colletotrichum gloeosporioides using a PCR-based approach. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 289-295 © TÜBİTAK doi: Molecular detection of antibiotic related genes from Pseudomonas aeruginosa FP6, an antagonist towards Rhizoctonia solani and Colletotrichum gloeosporioides Sasirekha BAKTHAVATCHALU, Srividya SHIVAKUMAR*, Shankar Bhat SULLIA Department of Microbiology, Centre for PG Studies, Jain University, Bangalore, India Received: Accepted: Published Online: Printed: Abstract: Despite the importance of antibiosis in biological control, little is known about the genes involved in antifungal activity. Therefore, the present study was aimed at identifying the location of the antagonistic gene(s) in Pseudomonas aeruginosa FP6 towards Rhizoctonia solani and Colletotrichum gloeosporioides using a PCR-based approach. A new bacterial strain, designated as FP6, was isolated from rhizospheric soil and identified as a member of Pseudomonas aeruginosa based on 16S rRNA analysis. The secondary metabolites produced by this strain have shown broad-spectrum antifungal activity against Rhizoctonia solani and Colletotrichum gloeosporioides. Antifungal metabolites of a nonenzymatic nature were found to be responsible for the antagonism. Hyphal malformation was also observed in both of the fungal pathogens. PCR analysis of the genomic DNA with antibiotic specific primers detected phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, and pyoluteorin. Plasmid isolation and plasmid curing of the strain exhibited varied antagonistic activity. A chromosomal gene was found to be involved in the production of a fungal antagonistic compound as demonstrated by the gel elution technique using antibiotic gene-specific primers. The study speculates that antimetabolites play an important role in the control of phytopathogens. Key words: Fluorescent pseudomonads, PCR, Rhizoctonia solani, Colletotrichum .

• Sinh học
• Fluorescent pseudomonads
• Rhizoctonia solani
• Colletotrichum gloeosporioides
• Plasmid curing
• Polymerase chain reaction
• Sclerotium rolfsii
• Collor or root rot
• Soybean caused
• Fluorescent Pseudomonads against collor
• Agricultural science
• Fluorescent pseudomonads isolates
• The growth promotion of tomato plant
• Soil borne pathogens
• Seed bacterization
• Defense induction
• Growth promotion of Jute
• Seed bacterization with fluorescent pseudomonads
• Medicinal plants
• Talcbased information
• Disease management
• Ashwagandha root diseases
• Maize leaf blights
• Helminthosporium turcicum
• Bipolaris maydis
• Systemic fungicides
• Disease severity
• Pseudomonas fluorescens against sheath blight
• Pseudomonas fluorescens against sheath blight in rice
• Screening PGPR
• Bacillus spp
• Plant growth promoting traits
• Coconut (Cocos nucifera L )
• AM fungi
• Arbuscular mycorrhizal fungi
• Chilli rhizosphere microorganisms
• Population density
• Glomus macrocarpum
• Bài giảng Polymerase Chain Reaction
• Taq polymerase
• Enzyme bền nhiệt
• Thiết kế primer
• PCR cải biến
• Bombyx mori L
• Grasserie virus
• Detection of grasserie virus
• Bovine papilloma
• Polymerase chain reaction in cattle
• Detection of bovine papillomavirus
• Bovine papillomavirus in cutaneous lesions
• Medicinal and aromatic plants
• Rapid DNA isolation
• Secondary metabolites
• Subsequent polymerase chain reaction
• L monocytogenes
• Chicken meat
• Areas of chennai
• Microbial quality (Listeria monocytogenes)
• Chicken meat by polymerase chain reaction
• Diagnosis of equine herpes virus
• Equine herpes virus 4 infection
• Horses showing symptoms of respiratory disease
• Tạp chí y học
• Nghiên cứu y học
• Mycobacterium tuberculosis và mycobacterirum khác mycobacterium tuberculosis
• Bệnh lý lao da
• Phản ứng chuỗi polymerase
• kỹ thuật PCR
• DNA
• phương pháp khuếch đại đoạn DNA
• nghiên cứu sinh học
• sinh học
• bài giảng sinh học
• Luận văn Thạc sĩ Sinh học
• Ứng dụng phương pháp PCR
• Sự nhiễm vi sinh vật gây bệnh
• Thực phẩm đường phố
• VSV gây bênh trong TP đường phố
• C perfringens trong thực phẩm đường phố
• certification of conventional soybean seeds and grains
• detection of transgenic soybean
• polymerase chain reaction (PCR)
• Roundup Ready™ (RR™) soybean
• Virus cúm gia cầm A H5N1
• Kỹ thuật Multiplex Reverse Transcription Polymerase Chain Reaction
• Luận văn thạc sĩ khoa học
• Virus cúm A
• Sinh học thực nghiệm
• nhuộm Haematoxylin và Eosin
• luận văn thủy sản
• nuôi trồng thuỷ sản
• giải pháp phát triển
• ngành thủy sản
• chuyên đề thực tập
• Tạp chí khoa học
• Tạp chí y dược
• Y dược quân sự
• Kỹ thuật reverse transcription polymerase chain reaction
• Xác định virut Ebola
• Định lượng hepatitis B virus DNA
• Kỹ thuật real time polymerase chain reaction
• Nhiễm virus viêm gan B
• Týp gen caga và vaca
• Phương pháp multiplex polymerase chain reaction
• Viêm dạ dày
• Bacterial vaginosis
• Multiplex polymerase chain reaction
• Bacterial vaginosis detection
• Serious complications affecting
• Long term health of women
• BMC Pediatrics
• HIV 1 infection
• Filter paper dried blood spots
• HIV 1 diagnosis
• Early diagnosis
• Early diagnosis of leptospirosis
• Clinical diagnosis
• Leptospirosis during
• Presence of campylobacter jejuni
• Cross contamination
• Food borne bacterial illness
• Antibiotic treatment
• Rapid enterovirus polymerase chain reaction
• Aseptic meningitis
• Causative viral infections
• Mycobacterium avium subspecies paratuberculosis
• Nested PCR
• ZiehlNeelsen staining
• Nested polymerase chain reaction
• Diagnosis of paratuberculosis
• Theileria annulata
• Cattle calves
• Cross bred
• Breed related hospital prevalence
• Theileria annulata infection in cattle calves confirmed
• Enzyme linked immunosorbent assay
• CMV DNA
• Pregnant women
• Detection of cytomegalovirus
• PALCAM selective agar
• BHI brothand PCR
• Molecular polymerase chain reaction
• Employing conventional isolation techniques
• Klebsiella oxytoca
• Molecular diagnosis
• Polymerase chain reaction targeted
• polygalacturonase gene
• Molecular discrimination of klebsiella oxytoca