TAILIEUCHUNG - Construction of dense genetic linkage maps of apple cultivars Kaşel-41 and Williams’ Pride by simple sequence repeat markers

Almost all commercially grown apple cultivars in Turkey are exotic, with local varieties serving as important genetic resources for breeding and marker development purposes. Among the latter is Amasya, which has a pleasing taste and flavor, but is unsuitable for commercial use because of its small fruit size and alternate-bearing characteristics. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 967-975 © TÜBİTAK doi: Research Article Construction of dense genetic linkage maps of apple cultivars Kaşel-41 and Williams’ Pride by simple sequence repeat markers 1 1, 2 Elmira ZIYA MOTALEBIPOUR , Salih KAFKAS *, Şerif ÖZONGUN , Ayşe Nilgün ATAY 1 Department of Horticulture, Faculty of Agriculture, Çukurova University, Adana, Turkey 2 Eğirdir Fruticulture Research Institute, Isparta, Turkey Received: Accepted/Published Online: 2 Printed: Abstract: Almost all commercially grown apple cultivars in Turkey are exotic, with local varieties serving as important genetic resources for breeding and marker development purposes. Among the latter is Amasya, which has a pleasing taste and flavor, but is unsuitable for commercial use because of its small fruit size and alternate-bearing characteristics. Genetic maps of apple cultivars are important resources to facilitate marker-assisted selection. Because of its good eating quality, Amasya is a good genetic resource for marker development to enhance fruit quality, especially flavor. In this study, we constructed a simple sequence repeat (SSR) marker-based genetic map of the Amasya apple cultivar using 96 F1 progenies generated from a cross between Kaşel-41 (a selection from Amasya) and Williams’ Pride cultivars. Of 187 bacterial artificial chromosome SSRs, 150 expressed sequence tag SSRs, and of 362 genomic SSR primer pairs, 400 markers were mapped, with parental and consensus maps constructed independently. A reference map was also constructed using 207 common SSR markers, thereby producing the most dense SSR-based reference map currently reported for apple. The reference genetic map was 1351 cM long, with a mean marker density of cM. The consensus map obtained by mapping 396 markers had a length of cM and a mean marker density. .

• Nông nghiệp
• Apple cultivars Kaşel 41
• Simple sequence repeat
• Genetic mapping
• Genetic linkage maps
• Apple cultivars in Turkey