TAILIEUCHUNG - Generation of transgenic sugar beet (Beta vulgarism L.) overexpressing the polygalacturonase inhibiting protein 1 of Phaseolus vulgaris (PvPGIP1) through Agrobacterium-mediated transformation

Numerous diseases caused by fungal pathogens influence the annual production of sugar beet. In order to obtain a plant resistant to fungi, genetic transformation has been applied to the sugar beet. To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes (CWDEs), polygalacturonases (PGs) are pathogenicity factors produced at the earlier stages of a fungal infection that depolymerize the homogalacturonan. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 429-438 © TÜBİTAK doi: Research Article Generation of transgenic sugar beet (Beta vulgarism L.) overexpressing the polygalacturonase inhibiting protein 1 of Phaseolus vulgaris (PvPGIP1) through Agrobacterium-mediated transformation 1,2 2, 2 2 Reza MOHAMMADZADEH , Mostafa MOTALLEBI *, Mohammadreza ZAMANI , Zahra MOGHADDASSI JAHROMI , 3 4 4 Peyman NOROUZI , Manuel BENEDETTI , Gilulia DE LORENZO 1 Department of Cell and Molecular Biology, Faculty of Science, Maragheh University, Maragheh, Iran 2 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 3 Sugar Beet Seed Institute, Karaj Iran 4 Pasteur Fondazione Cenci Bolognetti Institute, “C. Darwin” Department of Biology and Biotechnology, Sapienza University of Rome, Rome, Italy Received: Accepted: Published Online: Printed: Abstract: Numerous diseases caused by fungal pathogens influence the annual production of sugar beet. In order to obtain a plant resistant to fungi, genetic transformation has been applied to the sugar beet. To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes (CWDEs); polygalacturonases (PGs) are pathogenicity factors produced at the earlier stages of a fungal infection that depolymerize the homogalacturonan. One of the strategies used by plants to limit the degradation of the cell wall polysaccharides by fungal CWDEs is the production of proteinaceous inhibitors. Against fungal, microbial, and insect PGs, plants produce cell wall-associated polygalacturonase-inhibiting proteins (PGIPs). The overexpression of PGIPs improves the resistance to fungal and bacterial necrotrophs in different plants. In this research, the gene encoding the PGIP1 fused downstream of the leader sequence for secretion in the extracellular environment was isolated from Phaseolus

• Nông nghiệp
• Generation of transgenic sugar beet
• Plant transformation
• Phaseolus vulgaris
• Phytopathogenic fungi
• Polygalacturonase inhibiting proteins