TAILIEUCHUNG - The effects of DNA methyl transferases on antiaging klotho gene expression

Several intracellular metabolic pathways in which multiple genes have a role affect the aging process. One important genetic factor associated with aging is klotho gene. In some recently published studies, klotho gene has also been associated with several types of cancer and identified as a tumor suppressor gene | Turkish Journal of Biology Research Article Turk J Biol (2016) 40: 797-806 © TÜBİTAK doi: The effects of DNA methyl transferases on antiaging klotho gene expression Elif ÇAĞLAYAN, Kadir TURAN* Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Marmara University, İstanbul, Turkey Received: Accepted/Published Online: Final Version: Abstract: Several intracellular metabolic pathways in which multiple genes have a role affect the aging process. One important genetic factor associated with aging is klotho gene. In some recently published studies, klotho gene has also been associated with several types of cancer and identified as a tumor suppressor gene. Therefore, elucidation of the control of klotho gene expression has become more important. Results suggesting that the promoter region of human klotho gene could be epigenetically controlled by DNA methylation have been reported. In contrast, a study revealing whether a change in the expression level of DNMT enzymes in the cells affects klotho gene expression has not been found. Herein, the effects of DNMT3A and DNMT3B enzymes on the expression of klotho gene, an important genetic factor relating to the aging process and some human cancer types, were investigated. For this purpose, the expression levels of DNMT3A and DNMT3B in HEK293 cells were artificially changed, and the effect on klotho gene promoter activity was investigated using a reporter gene. The results of this research showed that DNMT enzymes have negative regulatory effects on klotho gene promoter organized as a chromatin structure, and they have an enhancing effect on promoter activity when it is located on plasmid DNA. These results elucidate the control mechanism of human klotho gene expression. Key words: Aging, DNA methylation, DNMT-3A, DNMT-3B, klotho 1. Introduction The aging process is determined by both environmental and genetic .

• Sinh học
• DNA methylation
• Antiaging klotho gene expression
• Multiple genes
• Klotho gene
• Klotho promoter methylation
• BMC Cancer
• Breast cancer
• Methylation index
• Aberrant DNA methylation
• Genome Biology
• Non coding RNA
• ATP dependent chromatin remodeling
• RNA directed DNA methylation
• Several tested loci
• Genome wide DNA Methylation
• 5 MC quantification
• Methylation specific PCR
• Paediatric tuberculosis
• Genome wide global DNA methylation
• Germ Cell Tumor
• Epigenetic reprogramming
• Pluripotency related pathways
• Determining cellular identity
• Environmental responses
• Forcible methylation
• DNA epigenetic modifier enzymes
• BMC Genomics
• DNA integrity
• DNA fragmentation index
• Paternal genome
• Sperm DNA integrity
• BMC Bioinformatics
• Bisulfite sequencing
• DNA methylation patterns
• Methylation pattern
• Differential methylation analysis
• Downstream analysis
• Illumina Infinium methylation arrays
• Gene expression
• Non CpG methylation
• Prenatal homogenate cortex
• Cellular identity
• Drought Stress
• DNA analysis
• Drought stress in maize
• Differential DNA methylation
• Methylation sensitive high resolution melting analysis
• Cystatin M
• Methylation specific PCR
• Phenotypic plasticity
• Genomic data
• Sociality through DNA Methylation
• Epigenetic control of sociality
• Prostate cancer
• Current diagnostic tools
• Non invasive diagnostic biomarker
• Allele specifc DNA methylation
• Alle lespecifc gene expression
• Eukaryotic organisms
• BMC Pulmonary Medicine
• Lung function
• Peripheral blood
• DNA methylation profiles
• Chronic obstructive pulmonary disease
• Endometrial stromal cells
• RNA expression
• Histone modification
• Bisulfite sequencing analysis
• Methylation co occurrence patterns
• Allele specific methylation
• Single locus modeling
• Precise prediction
• Logistic regression
• Differential methylation
• Sexual dimorphism
• Tissue specific methylation
• Cytosine methylation
• Spontaneous epiallele
• Epigenetic map
• Trans chromosomal methylation
• Methylation sensitive amplification polymorphism
• Tissue specificity
• Synergistic effect
• Nuclear DNA
• DNA methylation of alu repeats
• Complicated urinary tract infection
• Alu repeats
• Ethylation specific polymerase chain reaction
• Atopic asthma
• Gene expression profile
• DNA methylation profile
• DNA methylation/CpGs
• Gene ontology
• scientific reports
• thesis report
• medical report
• DNA methylation affects important developmental processes in both plants and animals
• The process of methylation of cytosines at C 5 is catalysed by DNA methyltransferases (MTases)
• which are highly conserved
• both struc turally and functionally
• in eukaryotes
• In this study
• we identified and characterized cytosine DNA MTase genes that are activated with the onset of reproductive development in rice
• 5 methylcytosine
• DNA damage
• Neuroimaging techniques
• Collect tumor tissue