TAILIEUCHUNG - Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability
An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. | Turkish Journal of Biology Turk J Biol (2016) 40: 781-796 © TÜBİTAK doi: Research Article Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability 1, 2 3 4 5 Ahmet ONAY *, Engin TİLKAT , Veysel SÜZERER , Özge KARAKAŞ METİN , Yelda ÖZDEN ÇİFTÇİ , 1 5 6 7 Fatih Mehmet KILINÇ , İbrahim KOÇ , Muhammet ŞAKİROĞLU , Hakan YILDIRIM , 8 1 1 Ahu ALTINKUT UNCUOĞLU , Nazan ÇALAR , Ömer Faruk AKDEMİR 1 Department of Biology, Faculty of Science, Dicle University, Diyarbakır, Turkey 2 Department of Biology, Faculty of Science, Batman University, Batman, Turkey 3 Department of Medical Services and Techniques, Bingöl University, Bingöl, Turkey 4 Genetic Engineering and Biotechnology Institute, Marmara Research Center, TÜBİTAK, Gebze, Kocaeli, Turkey 5 Department of Molecular Biology and Genetics, Gebze Institute of Technology, Gebze, Kocaeli, Turkey 6 Department of Bioengineering, Faculty of Engineering, Kafkas University, Kars, Turkey 7 Department of Horticulture, Faculty of Agriculture, Dicle University, Diyarbakır, Turkey 8 Department of Bioengineering, Faculty of Engineering, Marmara University, Göztepe, İstanbul, Turkey Received: Accepted/Published Online: Final Version: Abstract: An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. Since rooting of the regenerated shoots for both genotypes was not achieved, an in vitro micrografting method was developed for the production of plantlets. Moreover, genetic stability of 3-, 6-, and 24-times subcultured clones of both genotypes was assessed and compared with the mother plants using fluorescent-based amplified fragment length polymorphism (AFLP) analysis. The set of main plants and the different subcultured .
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