TAILIEUCHUNG - Enhancement of chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells using TGF-β3 and BMP-6

This study was designed to evaluate the in vitro chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells (AT-MSCs) in response to different culture conditions. | Turkish Journal of Biology Turk J Biol (2016) 40: 360-368 © TÜBİTAK doi: Research Article Enhancement of chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells using TGF-β3 and BMP-6 1 1,2, 1,2 Milad SHADEMAN , Abbas PARHAM *, Hesam DEHGHANI Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 2 Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran 1 Received: Accepted/Published Online: Final Version: Abstract: This study was designed to evaluate the in vitro chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells (AT-MSCs) in response to different culture conditions. Fat tissue cells after collection were cultured in optimized conditions until passage 3 (P3). P3 cells were cultured as micropellets under different chondrogenic conditions for 21 days in 5 groups: basic medium as a control, basic chondrogenic medium (BCM), BCM supplemented with transforming growth factor beta 3 (TGF-β3; 10 ng/mL), BCM supplemented with bone morphogenetic protein 6 (BMP-6; 10 ng/mL), and BCM supplemented with both TGF-β3 and BMP-6. The growth rate of pellets was measured and differentiation progression was assessed by specific stainings and gene expression profiling. Results showed that the largest pellets of cells belonged to the group containing both growth factors. All treated cells showed different levels of chondrogenic differentiation compared to the control group (P ) greater than the group with only TGF-β3 (Table 2). Table 1. Nucleotide sequences of the primer sets used for RT-PCR. Genes GenBank accession number Primer pairs Annealing temperature (°C) Amplicon size (bp) Equine GAPDH NM_001163856 F: TGTCATCAACGGAAAGGC R: .

• Sinh học
• Mesenchymal stem cells
• Cchondrogenic differentiation
• TGF β3
• BMP 6
• Adipose tissue derived MSCs
• Inhibited growth of mesenchymal stem cells
• Mesenchymal stem cells under simulated microgravity
• Simulated microgravity
• Cyclin dependent kinase 6
• Regenerative medicine
• Bone regeneration
• Dental pulp stem cells
• Human periapical cysts mesenchymal stem cells
• hPCy MSCs
• BMC Veterinary Research
• Multipotent mesenchymal stromal cells
• Neurodegenerative diseases
• Neural cellular lineages
• BMC Cancer
• Type 35 adenovirus fiber
• Human mesenchymal stem cells
• Coxsackie adenovirus receptor
• Histone methyltransferase
• Mesenchymal stromal cells
• Stem cells
• Human bone marrow mesenchymal stromal cells
• Induced pluripotent stem cells
• Dendritic cells
• Exosomes released
• Umbilical cord derived mesenchymal
• Mature dendritic cells
• Transfection efficiency
• Pluronic PF68
• Transfect human tooth germ stem cells
• Oxidative stress
• Oxidative stress plays
• Anti inflammatory
• Ovarian cancer
• Platelet activating factor
• Tumour microenvironment
• Turkish Journal of Biology
• Tạp chí Sinh học
• 5 10 Methyltetrahydrofolate reductase
• Human bone marrow derived mesenchymal stem cells
• Enzyme methionine synthase
• Scaffolds from medical grade chitosan
• 3D cultivation of mesenchymal stem cells
• Medical grade chitosan
• Rat adipose tissue derived mesenchymal stem cells
• Beta cells
• Insulin producing cells
• Mesenchymal epithelial transition
• Transcription factors
• BMC Molecular and Cell Biology
• Mesenchymal stem cell
• Cell surface markers
• Bone marrow mesenchymal stem cell
• Breast adipose stem cell
• Fibroblasts and olfactory
• Embryonic stem cells
• Stem cell based therapy
• Human embryonic stem cell
• Journal of Biology
• Endothelial cells
• Fluid shear stress
• Integrin β1
• Human umbilical vein endothelial cells (HUVECs)
• Reactive oxygen species
• Antioxidant enzymes
• Osteogenic differentiation
• Bone health
• Dental bud stem cells
• Non pharmacological therapies
• Recombinant proteins
• Conditioned medium
• Hematopoietic stem cell
• Mesenchymal stem
• Stromal cells
• Progenitor cells capable
• Homogeneous progenitor cell
• Bone defect
• Bone marrow mesenchymal stem cells
• Fibrous scaffold
• Three dimensional mesenchymal stem cell
• Biocompatible polyurethane
• Cardiomyocyte like cell
• Nerve growth factor
• Ewing sarcoma
• Mouse model
• Mesenchymal origin
• Three dimensional cell culture
• Umbilical cord mensenchymal stem cells
• Hepatocellular carcinoma
• Glioma stem cell
• Cell fusion
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• Umbilical cord
• Hematopoietic stem cell transplantation
• Graft versus host disease
• Wharton’s jelly