TAILIEUCHUNG - Molecular characterization of edible pea through EST-SSR markers
The genetic diversity among 23 newly developed homosegregate pea lines (Pisum sativum L.) was assessed with a total of 13 expressed sequence tag (EST) based-simple sequence repeat (SSR) markers. The percentages of amplified and nonamplified primers were 92% and 8%, respectively, and of the used primers gave the PCR product within the reported size range while of primers gave a different product size. | Turkish Journal of Botany Turk J Bot (2017) 41: 338-346 © TÜBİTAK doi: Research Article Molecular characterization of edible pea through EST-SSR markers 1 2 1 2 3, Mohammad NISAR , Asaf KHAN , Syed Fazal WADOOD , Aftab Ali SHAH , Fatih HANCI * 1 Department of Botany, University of Malakand, Khyber Pakhtunkhwa, Pakistan 2 Department of Biotechnology, University of Malakand, Khyber Pakhtunkhwa, Pakistan 3 Atatürk Central Horticultural Research Institute, Yalova, Turkey Received: Accepted/Published Online: Final Version: Abstract: The genetic diversity among 23 newly developed homosegregate pea lines (Pisum sativum L.) was assessed with a total of 13 expressed sequence tag (EST) based-simple sequence repeat (SSR) markers. The percentages of amplified and nonamplified primers were 92% and 8%, respectively, and of the used primers gave the PCR product within the reported size range while of primers gave a different product size. Polymorphism information content (PIC), major allele frequency, and variation in genetic diversity were calculated. The PIC ranged from to with an average of . Major allele frequency ranged from to with a mean value of . The variation in genetic diversity among these pea lines ranged from to with a mean value of . Cluster analysis based on a dendrogram divided the 23 pea lines into two main groups (L-1 and L-2), separated at 25% genetic distance. Seven subclusters were evident from these two main groups. L-1 grouped (12 pea lines) while L-2 contained (11 pea lines) of the total analyzed population. It was concluded that EST-SSR markers are useful for refinement of the pea linkage map. Key words: Pea, expressed sequence tags, cluster analysis, genetic diversity, polymorphism information content 1. Introduction Economically, legumes are the second best-known crop family with .
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