TAILIEUCHUNG - Antifungal activity of chitinase II against Colletotrichum falcatum Went. causing red rot disease in transgenic sugarcane

We evaluated transgenic lines of sugarcane modified with the barley chitinase class-II gene to create resistance against the red rot causative agent Colletotrichum falcatum Went. | Turkish Journal of Biology Turk J Biol (2018) 42: 45-53 © TÜBİTAK doi: Research Article Antifungal activity of chitinase II against Colletotrichum falcatum Went. causing red rot disease in transgenic sugarcane 1 2 1, 1 Muhammad TARIQ , Anwar KHAN , Bushra TABASSUM *, Nida TOUFIQ , 1 1 1 1 Muhammad Umar BHATTI , Saman RIAZ , Idrees Ahmad NASIR , Tayyab HUSNAIN 1 Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan 1 Department of Genetics, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan Received: Accepted/Published Online: Final Version: Abstract: We evaluated transgenic lines of sugarcane modified with the barley chitinase class-II gene to create resistance against the red rot causative agent Colletotrichum falcatum Went. Local sugarcane cultivar SP93 was transformed with a 690-bp coding sequence of the chitinase-II gene under the influence of a polyubiquitin promoter. Transgenic sugarcane lines (T0) overexpressing the chitinase gene were obtained through a particle bombardment method with transformation efficiency. Four transgenic sugarcane lines, SCT–03, SCT–05, SCT–15, and SCT–20, were tested for resistance against red rot by in vitro antifungal assays. Crude protein extracts from transgenic sugarcane plants SCT–03, SCT–05, SCT–15, and SCT–20 inhibited the mycelial growth of C. falcatum by 49%, 40%, 56%, and 52%, respectively, in a quantitative in vitro assay. Our findings revealed that two transgenic lines, SCT–15 and SCT–20, exhibited the highest endochitinase activity of and U/mL, respectively. Furthermore, transgenic lines SCT–15 and SCT–20 exhibited strong resistance against inoculated C. falcatum in an in vitro bioassay, as they remained healthy and green in comparison with the control sugarcane plants, which turned yellow and eventually died 3 weeks after infection. The mRNA expression of the .

• Sinh học
• Red rot tolerant sugarcane
• Antifungal gene
• Colletotrichum falcatum Went
• Sugarcane transformation
• Chitinase gene
• Chitinase activity
• Transgene mRNA expression