TAILIEUCHUNG - Synergistic effect of 24-epibrassinolide and salicylic acid on photosynthetic efficiency and gene expression in Brassica juncea L. under Pb stress
Lead (Pb) has wide-ranging effects on various essential physiological processes in plants including seed germination, root/shoot growth, photosynthetic efficiency, water status, and activities of enzymes. | Turkish Journal of Biology Turk J Biol (2017) 41: 943-953 © TÜBİTAK doi: Research Article Synergistic effect of 24-epibrassinolide and salicylic acid on photosynthetic efficiency and gene expression in Brassica juncea L. under Pb stress 1 1 2 1 1 1, Sukhmeen Kaur KOHLI , Neha HANDA , Anket SHARMA , Vinod KUMAR , Parminder KAUR , Renu BHARDWAJ * 1 Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India 2 Department of Botany, DAV University, Sarmastpur, Jalandhar, India Received: Accepted/Published Online: Final Version: Abstract: Lead (Pb) has wide-ranging effects on various essential physiological processes in plants including seed germination, root/ shoot growth, photosynthetic efficiency, water status, and activities of enzymes. The effect of combined treatment of 24-epibrassinolide (10–7 M, EBL) and salicylic acid (1 mM, SA) on growth, photosynthetic attributes, and phenolic compounds in 30-, 60-, and 90-day-old plants of Brassica juncea L. under Pb stress ( mM, mM, and mM) were studied. It was observed that Pb toxicity resulted in lowered growth and photosynthetic efficiency. The expressions of CHLASE, PSY, CHS, and PAL genes were altered. Presoaking treatment with the combination of EBL and SA for metal-stressed plants mitigated the adverse effects of metal stress by improving growth and levels of pigment and phenolic compounds. Key words: Brassinosteroids, chlorophyll, qRT-PCR, root growth, CHLASE gene 1. Introduction Photosynthesis is an imperative biological phenomenon, which bestows growth and development of plants. It converts light energy to useful chemical energy required for many metabolic processes (Pan et al., 2012; Ashraf and Harris, 2013). Disturbed photosynthesis and alteration of the ultrastructure of chloroplasts are prime symptoms of metal toxicity. Metal ions restrict the functioning .
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