TAILIEUCHUNG - Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro: A study emphasizing signaling molecule expression and transcriptional changes

Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. | Turkish Journal of Biology Turk J Biol (2017) 41: 785-795 © TÜBİTAK doi: Research Article Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro: a study emphasizing signaling molecule expression and transcriptional changes 1 2 1 1 1, Qiong WU , Chang-xin JIN , Hui CHEN , Xue-yong LI , Yue-jun LI * Department of Plastic and Burn Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi, . China 2 Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shanxi, . China 1 Received: Accepted/Published Online: Final Version: Abstract: Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migrationrelated regulators in MCF-7 cells were significantly enhanced in both protein (Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin) and mRNA (SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin) levels. The expression levels of oncogenes (RAS and HER-2) and an antioncogene gene (P53) in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and .

• Sinh học
• Adipose derived stem cells
• Functional proteins
• Carcinoma associated cells
• Tumor microenvironments
• Antioncogene gene
• Human adipose derived stem cells
• Adipose tissue
• Hyaluronic acid
• Soft tissue augmentation
• Human specific Alu gene
• BMC Veterinary Research
• Mesenchymal stem cells
• Cerebrospinal fluid
• Synovial fluid
• Plasmaderived exosomes