TAILIEUCHUNG - Comparison of a ceramidase inhibitor (ceranib-2) with C2 ceramide and cisplatin on cytotoxicity and apoptosis of glioma cells

Inhibiting ceramidase activity in cancer cells has been identified as a promising target for cancer therapy in recent studies. Thus, we examined the possible role of ceranib-2, a novel ceramidase inhibitor, on growth and apoptotic mechanisms of the human normal glia cell line (HNA), human glioma cell lines (T-98G and U-87MG), and a rat glioma cell line (C6). | Turkish Journal of Biology Turk J Biol (2018) 42: 259-265 © TÜBİTAK doi: Research Article Comparison of a ceramidase inhibitor (ceranib-2) with C2 ceramide and cisplatin on cytotoxicity and apoptosis of glioma cells 1, 2 3 2 2 Gökhan KUŞ *, Mete ÖZKURT , Pınar ÖZTOPCU VATAN , Nilüfer ERKASAP , Ruhi UYAR , Selda KABADERE 1 Department of Health Programs, Open Education Faculty, Anadolu University, Eskişehir, Turkey 2 Department of Physiology, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey 3 Department of Biology, Faculty of Arts and Sciences, Eskişehir Osmangazi University, Eskişehir, Turkey Received: Accepted/Published Online: 2 Final Version: Abstract: Inhibiting ceramidase activity in cancer cells has been identified as a promising target for cancer therapy in recent studies. Thus, we examined the possible role of ceranib-2, a novel ceramidase inhibitor, on growth and apoptotic mechanisms of the human normal glia cell line (HNA), human glioma cell lines (T-98G and U-87MG), and a rat glioma cell line (C6). We also compared the results with the effects of C2 ceramide and cisplatin. We determined the in vitro survival rate with MTT assay, apoptosis with flow cytometry, gene expressions with qRT-PCR, and statistical significance by one-way analysis of variance together with Tukey’s test. Calculated from MTT outcomes, the inhibitory ranking was as follows: T-98G > U-87MG > C6 > HNA. Ceranib-2 had the most growth-suppressive activity on human T-98G cells with an IC50 of 7 µM for 24 h and µM for 48 h. Only the 25 µM dose of ceranib-2 induced apoptosis of human T-98G and U-87MG cells after 24 h of treatment; however, it increased apoptosis of C6 cells dose- and time-dependently. Ceranib-2 increased the cytochrome c gene expression level during 24 h in T-98G cells. Ceranib-2 had cytotoxic and apoptotic effects on glioma cells but the .

• Sinh học
• Ceranib 2
• C2 ceramide
• Rat glioma cell line
• Human normal glia cell line
• Human glioma cell lines
• Journal of Chemistry
• Drug nanocarrier
• Magnetic nanocomposite
• Glioblastoma multiforme
• Malignant glioma
• Initial drug concentration