TAILIEUCHUNG - The Aggregatibacter actinomycetemcomitans heat shock protein GroEL interacts directly with human peripheral blood T cells

The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells. | Turkish Journal of Biology Research Article Turk J Biol (2016) 40: 1140-1147 © TÜBİTAK doi: The Aggregatibacter actinomycetemcomitans heat shock protein GroEL interacts directly with human peripheral blood T cells Ayten NALBANT*, Tahsin SAYGILI Department of Molecular Biology and Genetics, İzmir Institute of Technology, Urla, İzmir, Turkey Received: Accepted/Published Online: Final Version: Abstract: Heat shock family protein GroEL of Aggregatibacter actinomycetemcomitans (Aa) has antigenic properties. We previously demonstrated that A. actinomycetemcomitans GroEL-like protein affects human CD4 T cells by converting them into IL-10 and IFNg double cytokine producing Tbet+ Th1 cells. The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells. Expression of six different soluble cytokines was evaluated by CBA assay. To determine whether AaGroEL affects CD3+ T cells directly or not, purified CD3+ T cells or CD14+ cells were cultured with AaGroEL separately, and the quantity of soluble cytokine was measured. Results showed that sorted CD3+ cells produced soluble IL-6, TNFα, and IFNγ cytokines. Additionally, the intracellular cytokine staining data showed that AaGroEL-stimulated CD3+ cells were also TNFα- and IFNγ-positive. Moreover, AaGroEL-responsive T cells slightly increased their TLR2 expression. These findings suggest that CD3+ T cells produce cytokines in response to AaGroEL protein without requirements for other cells, such as CD14+ monocytes. Key words: T cells, Aggregatibacter actinomycetemcomitans GroEL, Hsp64, cytokines, TLR2 1. Introduction Aggregatibacter .

• Sinh học
• T cells directly
• Aggregatibacter actinomycetemcomitans
• Cytolethal distending toxin
• Hycult biotech
• Intracellular cytokine expression