TAILIEUCHUNG - Use of SNP markers by KASP assay for MAS studies in sunflower against Plasmopara halstedii
Downy mildew is a fungal disease caused by Plasmopara halstedii and leads to loss of yield up to 100% in sunflower. Disease control is performed mostly through chemical seed treatment and breeding. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2017) 41: 480-489 © TÜBİTAK doi: Use of SNP markers by KASP assay for MAS studies in sunflower against Plasmopara halstedii 1 1 2 3 1, Accepted/Published Online: Final Version: Kevser KÖSOĞLU , Sevcan YUMUK , Yıldız AYDIN , Göksel EVCİ , Ahu ALTINKUT UNCUOĞLU * 1 Department of Bioengineering, Faculty of Engineering, Marmara University, İstanbul, Turkey 2 Department of Biology, Faculty of Science and Arts, Marmara University, İstanbul, Turkey 3 Directorate of Trakya Agricultural Research Institute, Ministry of Food, Agriculture and Livestock, Edirne, Turkey Received: Abstract: Downy mildew is a fungal disease caused by Plasmopara halstedii and leads to loss of yield up to 100% in sunflower. Disease control is performed mostly through chemical seed treatment and breeding. Due to the time consuming nature of conventional breeding, it is supported by biotechnological approaches. Marker-assisted selection (MAS) is a strategic approach in molecular breeding using molecular markers. Single nucleotide polymorphisms (SNPs) such as insertions, deletions, and base-pair substitutions are more advantageous than other molecular markers. The abundance and biallelic nature of SNPs in a genome provide flexibility in the choosing of SNPs at the desired loci. Competitive allele-specific PCR (KASP) is a genotyping technology for screening of trait-specific SNP markers. In this study, SNP markers (NSA002867, NSA006138; NSA000052, NSA000354; NSA002220, NSA002251) linked with the downy mildew resistance genes Plarg, Pl13, and Pl8, respectively, were analyzed via KASP in three parental crosses (RHA-419 × Colombi, RHA-419 × P64LC53, RHA-419 × Oliva) for Plarg, one parental cross (HA-R5 × P64LC53) for Pl13, one parental cross (P64LC53 × HA-89) for Pl8, and 140 F2 individuals. According to the allelic .
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