TAILIEUCHUNG - Cloning and characterization of a serine/threonine protein phosphatase 2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam.

Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. | Turkish Journal of Biology Research Article Turk J Biol (2017) 41: 148-157 © TÜBİTAK doi: Cloning and characterization of a serine/threonine protein phosphatase 2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam. Pan XU, Bin YONG*, Huan-Huan SHAO, Jia-Bin SHEN, Bing HE, Qin-Qin MA, Xiang-Hua YUAN, Yu WANG College of Life Sciences, Sichuan Normal University, Chengdu, Sichuan, . China Received: Accepted/Published Online: Final Version: Abstract: Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites were distributed in the coding region of this gene, but most did not result in amino acid change. RNA sequencing-based digital gene expression profiling showed that this PP2A gene was primarily expressed in fibrous roots and developing tuberous roots under natural conditions. After drought, salinity, and alkaline stress treatment, the expression of IbPP2A1 was obviously upregulated in stems and tuberous roots at 2 days, whereas the expression of IbPP2A1 in leaves was only upregulated by drought stress at 2 days. However, heterogeneous expression of IbPP2A1 did not enhance abiotic stress tolerance in recombinant Saccharomyces cerevisiae. The results described here indicate that IbPP2A1 is an abiotic stress-responsive gene, but it could not .

• Sinh học
• Sweet potato
• Polymorphic site
• Expression pattern
• Stress resistance
• Protein phosphatase 2A
• Agronomic evaluation of sweet potato
• Agronomic evaluation
• Sweet potato (Ipomoea batatas L ) germplasm
• Related agronomic variation
• Quality evaluation
• Preparation of jam
• Sweet potato cultivarsSweet potato
• Evaluated physicochemically for ascorbic acid
• Yield of sweet potato
• Total storage root yield
• Day after planting
• Total carotenoid content
• Quality parameters
• Tuber yield
• Quality of sweet potato
• Bioregulators on growth
• Plant spacing
• Planting dates
• Yield attributes of sweet potato
• Plant spacing on growth
• Evaluation of physiochemical characters
• Physiochemical characters
• Physiochemical variation
• Specific gravity
• Potassium on yield
• Graded levels of nitrogen
• Post harvest profile
• Post harvest profile of sweet potato
• Processed food product
• Problems associated with improper curing
• Orange flesh sweet potato
• Genetic advance
• Orange flesh sweet potato genotypes
• Genetic variability
• Genetic advance for growth
• Value added food products
• Sensory evaluation
• Nutritional composition
• Potato peels
• Utilization of sweet potato peels
• Tạp chí khoa học và công nghệ
• Investigation of stability of colorant powders extracting
• Japanese purple sweet potato
• Ipomoea batatas (L
• ) Poir
• Preserve the colorant powder
• Purple sweet potato
• Date of planting
• Yield and economics
• Ipomoea batatas L
• Planting on growth
• Yield and economics of sweet potato
• Sweet potato juice
• Physical characteristics
• Potato juice variety bhu sona
• β Carotene
• Carotenoid content
• IbOr gene
• α carotene
• Sweet potato whitefly Bemesia tabaci Genn
• Culturable bacteria
• Erwinia persicinus
• Secondary symbionts
• Pseudomonas plecoglossicida
• White sweet potato
• Ipomoea batatas;
• Wound healing
• Re epithelialization
• Ulceration index
• Free radical scavenging
• Heat moisture treatment conditions
• Producing high amounts
• Resistant starches from purple
• Response surface methodology
• Roving survey
• Root knot nematode
• Major sweet potato
• Secondary infection
• Meloidogyne incognita
• Orange fleshed sweet potato
• Nitrogen and potassium
• Potassium on growth
• Yield and quality
• Yield parameters and quality parameters
• Ambient conditions
• Genotypes in retention
• Processing qualities under ambient conditions
• Genotypic coefficient
• Phenotypic coefficient of variation
• Sweet potato (Ipomoea batatas (L ) Lam)
• Hill zone of Karnataka
• Genetic variability studies
• BMC Plant Biology
• Storage root
• Plant hormone
• Plant growth regulators
• Quality characters
• Vegetative characters
• Plant growth regulators on growth
• Plant Biology
• Anthocyanin biosynthesis
• Regulatory complex
• Biological functions
• Full length transcriptome
• Transcription factor
• Weighted gene co expression network analysis