TAILIEUCHUNG - Bacterial diversity in penaeid shrimp hepatopancreas revealed by metagenome analysis

Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) is an emerging disease reported recently that has caused massive loss to saline shrimp aquaculture. EMS/AHPND was recognized in Vietnam since 2011 and up to now its adverse effect is still serious to shrimp industry nationwide. Determination of the main pathogenic factor and the role of other microbial candidates is a very important study. | Journal of Fisheries science and Technology Special issue - 2015 BACTERIAL DIVERSITY IN PENAEID SHRIMP HEPATOPANCREAS REVEALED BY METAGENOME ANALYSIS Hua Ngoc Phuc1, Truong Hai Nam2, Do Thi Huyen2, Nguyen Thi Trung2, Nguyen Thi Quy2, Duong Thu Huong2 ABSTRACT Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) is an emerging disease reported recently that has caused massive loss to saline shrimp aquaculture. EMS/AHPND was recognized in Vietnam since 2011 and up to now its adverse effect is still serious to shrimp industry nationwide. Determination of the main pathogenic factor and the role of other microbial candidates is a very important study. This report shares results of a metagenome analysis on bacterial diversity at community level that has been one of critical research efforts on EMS/AHPND in Vietnam in recent time. A total of 7 metagenomic samples were extracted from farmed penaeid shrimp hepatopancrea tissues including 2 samples of healthy shrimp populations and 5 others of infected shrimps at regions of 16S rDNA, recA, and rpoB were sequenced by Illumina HiSeq technology. The DNA metagenome analysis revealed a wide range of bacteria groups up to 63/171 genera on healthy and infected shrimp hepatopancreatic samples, respectively. The mainly dominant bacteria (percentage on healthy/infected samples, respectively) were Pseudomonas (), Stenotrophomonas (0/), Enterobacter (0/), and especially Vibrio (0/). The routine pathogens such as Aeromonas, Enterobacter, Samonella, Vibrio were not found in hepatopancreatic samples of healthy shrimps. Remarkable differences in this study compared to other previous reports will be discussed with a focus on the factor Vibrio parahaemoluticus and the mechanism of transmission in relevance to prevention of the disease. Keyword: penaeid shrimp, EMS/AHPND, metagenome, bacterial diversity I. INTRODUCTION An emerging disease occured with serious loss to shrimp aquaculture

• Ngư nghiệp
• Penaeid shrimp
• Early mortality syndrome
• Acute hepatopancreatic necrosis disease
• Bacterial diversity
• Bacterial DNA extraction
• Bacteria specific library construction
• Tạp chí Khoa học và Công nghệ Biển
• Detection and analysis of early genes
• White spot syndrome virus in penaeid shrimp
• Wsv303 and wsv477 genes
• Single step PCR