TAILIEUCHUNG - Indole acetic acid production by the indigenous isolates of azotobacter and fluorescent pseudomonas in the presence and absence of tryptophan

A total of 21 bacterial isolates (Azotobacter sp., 10 and fluorescent Pseudomonas sp., 11) were isolated from different rhizospheric soils in the vicinity of Aligarh city and characterized as per standard methods. These isolates were further tested for the production of indole acetic acid (IAA) in a medium with 0, 1, 2 and 5 mg/ml of tryptophan. | Turk J Biol 29 (2005) 29-34 © TÜB‹TAK Indole Acetic Acid Production by the Indigenous Isolates of Azotobacter and Fluorescent Pseudomonas in the Presence and Absence of Tryptophan Farah AHMAD, Iqbal AHMAD, Mohd Saghir KHAN Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh - 202002 INDIA Received: Abstract: A total of 21 bacterial isolates (Azotobacter sp., 10 and fluorescent Pseudomonas sp., 11) were isolated from different rhizospheric soils in the vicinity of Aligarh city and characterized as per standard methods. These isolates were further tested for the production of indole acetic acid (IAA) in a medium with 0, 1, 2 and 5 mg/ml of tryptophan. A low amount ( mg/ml) of IAA production was recorded by Azotobacter strains without tryptophan addition. Seven Azotobacter isolates showed high level ( to mg/ml) production of IAA at 5 mg/ml of tryptophan while at 1 and 2 mg/ml the production was in the range of to and to mg/ml, respectively. Production of IAA in fluorescent Pseudomonas isolates increased with an increase in tryptophan concentration from 1 to 5 mg/ml in the majority of isolates. In the presence of 5mg/ ml of tryptophan, 5 isolates of Pseudomonas produced high levels ( to mg/ml) of IAA while 6 other isolates produced IAA in the range of to mg/ml. Production of IAA was further confirmed by extraction of crude IAA from 3 isolates of Azotobacter (Azs1, Azs6 and Azs9 ) and three isolates of Pseudomonas (Ps1, Ps4 and Ps7) and subsequent TLC analysis. A specific spot from the extracted IAA preparation was found corresponding with the standard spot of IAA with same Rf value. Pseudomonas isolates (Ps1, Ps4 and Ps7) showed inhibitory effects on the growth of root elongation of Sesbania aculeata and Vigna radiata at all concentrations of tryptophan compared to the control. However, the isolates of Azotobacter (Azs1, Azs6 and Azs9) .

• Sinh học
• Indole acetic acid
• Fluorescent Pseudomonas sp
• Per standard methods
• Plant growth promoting rhizobacteria
• L tryptophan metabolism
• Trichoderma viride
• indole acetic acid produced
• Mangrove associated fungi trichoderma viride VKF3
• Fungal pathogens
• Ethyl methanesulphonate
• Gibberellic acid
• Somatic mutation
• Recombination test
• Drosophila melanogaster
• IAA producing bacteria
• Indole acetic acid producing bacteria
• Coasts of Ben Tre
• Tra Vinh provinces
• Producing bacteria could naturally stimulate
• Plant growth promotion
• TLC and L tryptophan
• Producing tomato
• Salt tolerant
• Abiotic stress
• Producing rhizobacteria
• Plant growth of mustard crop
• Salt stress condition
• Công nghệ nông nghiệp
• Bài viết về nông nghiệp
• Giống lúa HT1
• Nồng độ IAA
• Indole 3 acetic acid
• Aconitum heterophyllum
• Critically endangered
• Anther dehiscence
• Pollen germination
• Indole 3 butyric acid
• Naphthalene acetic acid
• Callus culture
• Epithelantha micromeris callus growth
• Effect of phytohormones
• Seed treatment
• Lactic acid bacteria
• Plant growth promoting
• Least Square Difference
• scientific reports
• Report medicine
• traditional medicine
• immune system
• Antimicrobial
• biosynthesis
• Plant growth promoting bacteria
• Nitrogen fixation
• Soil chemical properties
• Phosphate solubilisation
• Ultrasound accelerated arylthiomethylation
• Mannich type reactions
• Magnetic stirring and ultrasonic irradiation
• The mechanism and process
• Mannich type reactions in the synthesis
• Inorganic nutrients
• Zea mays
• Relative water content
• Salinity stressed maize
• Plant culture
• BMC Plant Biology
• Drought tolerance
• Antioxidant defense
• Tree species
• Trichoderma species
• Salt stress
• Wheat seedling
• Ionic toxicity
• Molar concentration
• Disease incidence
• Disease severity and yield
• Groundnut (Arachis hypogaea L )
• Potassium solubilization
• Plant growth
• Mustard (Brassica juncea L )
• Improve crop growth
• Phyllosphere microorganisms
• Phylloplane associated plant bacteria of rice
• Plant growth promoting activity
• Phylloplane of rice
• Methanol oxidizers
• N2 fixation
• Flooded paddy ecosystem harbors
• Methanol oxidizing plant growth promoting bacteria
• Order enterobacterales
• Cereal crops
• Maize rhizosperic phosphate solubilizing isolates
• Plant growth promoting characteristics
• Hydrogen cyanide production
• Indole acetic acid production
• In vitro biosolubilization
• Agricultural science
• Fungal endophytes associated
• Medicinal plant Asclepias sinaica
• Extracellular enzyme
• Medicinal plants