TAILIEUCHUNG - Characterization of salivaricin B, A protein expressed by Lactobacillus salivarius M7

Salivaricin B (SalB) is a protein encoded by Lactobacillus salivarius M7. This protein is insensitive to proteolytic enzymes, such as pepsin, trypsin, and chymotrypsin, but is partially sensitive to proteinase K. SalB is also stable and maintains its activity over a range of pHs, from 3 to 9. | Turk J Biol 27 (2003) 131-136 © TÜB‹TAK Characterization of Salivaricin B, A Protein Expressed By Lactobacillus salivarius M7 1 1 2 Osman ÇATALOLUK , Candan G. GÜRAKAN 2 Department of Medical Biology & Genetics, Faculty of Medicine, Gaziantep University, 27310, Gaziantep, Department of Food Engineering, The Middle East Technical University, 06610, Ankara - TURKEY Received: Abstract: Salivaricin B (SalB) is a protein encoded by Lactobacillus salivarius M7. This protein is insensitive to proteolytic enzymes, such as pepsin, trypsin, and chymotrypsin, but is partially sensitive to proteinase K. SalB is also stable and maintains its activity over a range of pHs, from 3 to 9. SalB was found to be effective against many closely related lactic acid bacteria such as, L. fermentum, L. plantarum, L. acidophilus and distantly related gram-positive bacteria, including Listeria monocytogenes, Streptococcus faecalis, Staphylococcus aureus and Staph. epidermidis. The mode of action of SalB was obviously bactericidal, and death occurs without bacterial lysis. The gene responsible for SalB production in L. salivarius M7 had previously been characterized. Key Words: Bacteriocin, Salivaricin B, Lactobacillus salivarius M7 Lactobacillus salivarius M7 Taraf›ndan ‹fade Edilen Salivarisin B Adl› Proteinin Karakterizasyonu Özet: Salivarisin B (Sal B) Lactobacillus salivarius M7 taraf›ndan kodlanan bir proteindir. Bu protein pepsin, tiripsin, kimotiripsin gibi protein parçalayan enzimlerden etkilenmemelerine ra¤men, di¤er bir protein parçalay›c› enzim olan proteinaz K’ya karfl› k›smen duyarl›d›rlar. Sal B 3 ile 9 aras› pH yelpazesinde aktiftir. Yap›lan çal›flmalarda, Sal B’nin Lactobacillus fermentum, Lactobacillus plantarum, ve Lactobacillus acidophilus gibi bir çok yak›n akraba bakteriler ve Listeria monocytogenes, Streptococcus faecalis, Staphylococcus aureus, ve Staphylococcus epidermidis gibi akraba olmayan bakteriler üzerine etkili oldu¤u bulunmufltur. Sal .

• Sinh học
• Salivaricin B
• Lactobacillus salivarius M7
• Proteolytic enzymes
• Lactic acid bacteria
• Tryptic soy broth
• Gene structure
• Inverse PCR
• Degenerate primers
• Lactobacillus salivarius M7
• Tạp chí y học
• Nghiên cứu y học
• Nuôi cấy bacillus
• Thực phẩm chức năng