TAILIEUCHUNG - Molecular characterization of the gene encoding for the salivaricin B activity and its flanking sequences

Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. | Turk J Biol 25 (2001) 379-386 © TÜB‹TAK Molecular Characterization of the Gene Encoding for the Salivaricin B Activity and its Flanking Sequences Osman ÇATALOLUK Gaziantep Üniversitesi, T›p Fakültesi, T›bbi Biyoloji Anabilim Dal›, 27310, Gaziantep - TURKEY Received: Abstract: Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and translocation of salivaricin B (SalB), a bacteriocin, from Lactobacillus salivarius M7 was studied at the molecular level. The gene encoding SalB and the flanking sequences were obtained and sequenced. The gene encoding SalB comprised an open reading frame (ORF) of 171 bp having a 57 bp long leader sequence and a 114 bp long structural part. Ribosomal binding site (GAGG, RBS) is located at a cannonical distance of 8 bp upstream from the start site. Key Words: Gene, Gene structure, Salivaricin B, PCR, Inverse PCR, Degenerate primers, Lactobacillus salivarius M7. Salivarisin B Aktivitesini Kodlayan Gen ve Çevre Nükleotid S›ralar›n›n Moleküler Karakterizasyonu Özet: Laktik asit bakterileri (LAB) baz›lar› g›da korunmas›nda kullan›lan birçok farkl› antibakteriyel protein üretirler. Bakteriosinlerin moleküler genetik analizleri son y›llarda oldukça ilgi çekmifl ve h›zlanm›flt›r. Buna paralel olarak bakteriosinlerin ekspresyon ve sal›n›mlar› rutin olarak incelenmeye bafllanm›flt›r. Bitki ve hayvan kökenli g›dalarla birlikte bulunduklar›ndan ortak amaç bu .

• Sinh học
• Gene structure
• Salivaricin B
• Inverse PCR
• Degenerate primers
• Lactobacillus salivarius M7
• BMC Bioinformatics
• Genome annotation
• Gene prediction
• Multiple sequence alignment
• Spliced alignment
• Ribosomal proteins
• Gene regulatory network
• Hub gene structure
• Ultra high dimensional problem
• Decomposable multi structure identification
• Influenza infection
• Gene expression data
• Gene network
• Simulate gene
• Stochastic simulation algorithm
• BMC Genomics
• Intron exon boundaries
• Glutamate synthases
• Gene expression
• Horizontal gene transfer
• Acquired genes
• Mobile genetic elements
• Physical linkage
• Association analysis
• Linear mixed models
• Structural annotation
• Manual annotation
• Manual curation
• Exon intron structure
• health
• environmental
• Viral Genomes
• Molecular Structure
• Diversity
• Gene
• Mechanisms
• Host Virus Interactions
• Maria Laura Garcia and Víctor Romanowski
• Plant Biology
• Genetic diversity
• Population genetic structure
• Gene flow
• Isolation by distance
• Tetraena mongolica Maxim
• BMC Genetics
• 3D genome structure
• Genetic variation
• Single nucleotide polymorphism
• Gene regulation
• Chromosome conformation capture
• Genome wide association study
• Gene fusions
• Gene fusion prioritization
• Chimeric transcript discovery tools
• RNA sequencing
• Eukaryotic genomes
• Unicellular organisms
• Multiple sequence alignments
• Homologous genes
• Abiotic stress
• Gene ontology
• Lipid phosphate phosphatases
• Stress responsive elements
• Genome Biology
• Transcription factors
• Lateral gene transfer
• Gene family evolution
• Evolution of development
• Report medicine
• traditional medicine
• scientific reports
• Antimicrobial
• cell structure
• genetics
• Crystal structure
• medical analysis
• biochemical studies
• Protein homology modelling
• KLF1 gene
• Ramchandran plot
• Insilco methodologies
• 3D structure prediction
• Genetic variability
• Genetic advance
• Additive gene
• Cucumber (Cucumis sativus L )
• Protected structure
• Genetic advance in cucumber
• Chromosome conformation
• Next generation sequencing
• Chromatin structure
• 3D structure
• Existing methods
• PAP enzymes
• Protein structure
• Phosphate use efficiency
• RNA secondary structure
• Untranslated Regions
• Coding DNA Sequence
• Non coding RNA
• Secondary structure
• Gene finding
• Pre aligned sequences
• oxidation
• bacteria
• Radiation hybrid mapping
• Transcription start site
• Whole mount in situ hybridization
• Puffer fish
• Yolk syncytial layer