TAILIEUCHUNG - The role of phospholiphase d in phospholiphid transaction during cotton seeds maturation

The role of phospholipase D (EC ) was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation. To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). | Turk J Biol 26 (2002) 241-246 © TÜB‹TAK The Role of Phospholiphase D in Phospholiphid Transaction During Cotton Seeds Maturation Muborak Mahmusovna ABDULLAEVA, Mamura Muhamedovna MULLADGANOVA, Mamlakat Mirzalievna UMAROVA Uzbekistan National University, Faculty of Biology, Department of Biochemistry, TASHKENT Received: Abstract: The role of phospholipase D (EC ) was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation. To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). Enzyme activity was judged from the formation of hydrolysis products - phosphatidic acid and phosphatidylmethanol. The phospholipid content was defined by using thin-layer chromatography with a follow up analysis of phosphorus quantity in phospholipid fractions. It was shown that phospholipase D displayed two functions during cotton seed maturation: transferase and hydrolase. Hydrolase function could be defined from the increase of the product (phosphatidic acid) concentration during the complete maturation of the seed. We noticed that the concentration increased. Transferase activity could be defined through the increase of the phosphatidylmetanol concentration during the incubation of phospholipids in a water-methanol environment. The experimental data showed that phospholipase D plays an important role in phospholipid metabolism. Key Words: Phospholipase D, Phospholipids, Cotton seeds. Introduction Much research has been conducted to investigate the phospholipid metabolism process and its enzymatic aspects (1-12). This process is very important in cell membrane regeneration and formation. As a result of a great deal of research, it was identified that quality and quantity changes took place in the structure of phospholipids during plant seed development because they undergo hydrolysis by phospholipase .

• Sinh học
• Phospholipase D
• Cotton seeds
• Seed homogenates
• Phospholipid fractions
• Hydrolysis phosphatidic acid
• Report medicine
• traditional medicine
• scientific reports
• Crystal structure
• medical analysis
• biochemical studies
• oxidation
• chemical reaction
• tumor cells
• molecular Biology
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• bacteria biochemical studies
• environmental medicine
• bacteria
• BMC Biotechnology
• Extracellular expression
• Cell autolysis
• Modified phospholipids
• Phospholipids modification
• BMC Plant Biology
• Transgenic tobacco
• Phosphatidic acid
• Drought stress
• Abscisic acid
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• Lipid peroxidation
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• Transformed phenotype