TAILIEUCHUNG - A new molecular technique for the identification of micro organisms in biological treatment plants: Fluorescent in situ hybridization

Wastewater treatment has gained increased importance worldwide due to increasing demand on fresh clean water supplies. Biological treatment achieved by the activities of micro-organisms, is one of the most widely used processes in the treatment of wastewater. | Turk J Biol 26 (2002) 57-63 © TÜB‹TAK Mini Review Article A New Molecular Technique for the Identification of Micro-organisms in Biological Treatment Plants: Fluorescent In Situ Hybridization Gülnur COfiKUNER Cumhuriyet University, Department of Environmental Engineering, 58140 Sivas - TURKEY Received: Abstract: Wastewater treatment has gained increased importance worldwide due to increasing demand on fresh clean water supplies. Biological treatment achieved by the activities of micro-organisms, is one of the most widely used processes in the treatment of wastewater. High numbers and activities of some groups of micro-organisms are required to treat wastewater in biological processes. In contrast, some groups of micro-organisms must be absent from the system since their presence and activities cause problems in the treatment of wastewater. The structure and function of micro-organisms in wastewater treatment plants (WWTP) have been investigated for decades. However, the identification of both the structure and function of these complex microbial communities is very limited with classical methods. Recently, it has become possible to identify micro-organisms with fluorescent in situ hybridization (FISH) in a cultivationindependent way. In this paper, how FISH is applied and the advantages and disadvantages of this method are considered. In addition, recent studies of FISH for characterizing micro-organisms in WWTP are discussed. Key Words: Wastewater, in situ hybridization, molecular method, microbial ecology Biyolojik Ar›tma Tesislerindeki Mikroorganizmalar›n Tan›s›nda Yeni Bir Moleküler Teknik: Yerinde Floresanl› Hibritleflme Özet: At›ksu ar›t›m› dünyada, temiz su kaynaklar›na artan ihtiyaçtan dolay› artan bir öneme sahiptir. Mikroorganizma gruplar›n›n aktiviteleri sonucu gerçekleflen biyolojik ar›t›m, günümüzde at›ksu ar›t›m›nda en çok kullan›lan proseslerden biridir. Biyolojik ar›tmada, at›ksuyun ar›t›m› için baz› mikroorganizma gruplar›n›n .

• Sinh học
• In situ hybridization
• Molecular method
• Microbial ecology
• Biological treatment
• Micro organisms
• BMC Developmental Biology
• Whole mount in situ hybridization
• Fluorescent in situ hybridization
• Tyramide signal amplification
• Multicolor FISH
• Peroxidase quenching
• Fluorescein in situ hybridization
• Real time PCR
• Hypersaline environments
• Çamaltı Saltern
• Polymerase chain reaction
• Tạp chí y học
• Nghiên cứu y học
• Kỹ thuật FISH
• Chẩn đoán đột biến gen elastin
• Hội chứng williams beuren
• Allium roseum L
• Basic chromosome number
• Ploidy level
• 5S and 45S rDNA
• Cereal crops
• Cultivated sorghum
• Genomic in situ hybridization
• Genomic relationship
• Sorghum halepense
• BMC Cancer
• Fluorescence in situ hybridization
• Clonal evolution
• Tyrosine kinase inhibitors
• Durable response rate
• Chronic myeloid leukemia
• BMC Genomics
• Satellite DNA
• Internal transcribed spacer
• Nuclear ribosomal DNA
• BMC Plant Biology
• E arundinaceus
• Suppression subtractive hybridization
• Species specific repetitive sequences
• Activated sludge flocs
• Ammonia oxidizing bacteria
• Confocal scanning laser microscopy
• Fluorescent in situ hybridization
• Biological oxidation
• Prostate cancer
• Genomic abnormalities
• Risk stratification
• Fluorescence in situ hybridisation
• Replication stress
• Oncogene induced senescence
• DNA damage response
• Lung cancer
• EMSY gene
• Cancer deaths
• Cell lung carcinogenesis
• Comparative genomic hybridization
• Situ hybridization
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• Báo cáo sinh học hay
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• tài liệu báo cáo nghiên cứu khoa học
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• cách làm luận văn
• cách trình bày luận văn
• hướng dẫn làm luận văn
• luận văn ngành công nghệ sinh học
• phương pháp insitu
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• Gene structure
• Radiation hybrid mapping
• Transcription start site
• Puffer fish
• Yolk syncytial layer
• BMC Pediatrics
• Williams syndrome
• Adaptive behavior
• Neurodevelopmental disease
• Dual translocation line
• Psathyrostachys huashanica
• Single nucleotide polymorphism array
• Wheat powdery mildew
• Gossypium hirsutum
• Gossypium anomalum
• Monosomic alien addition line
• Microsatellite marker
• Transposable element
• Chromosome structure
• Eukaryotic genomes