TAILIEUCHUNG - Production of monoclonal antibodies specific for progesterone, estradiole by simultaneous injection of different steroids

We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. | Turk J Biol 24 (2000) 697–705 © TÜBİTAK Production of Monoclonal Antibodies Specific for Progesterone, Estradiole by Simultaneous Injection of Different Steroids Fatıma ŞAHİNGÖZ YÜCEL Gen Mühendisliği ve Biyoteknoloji Araştırma Enstitüsü, TÜBİTAK Marmara Araştırma Merkezi, Gebze-Kocaeli-TURKEY Beyazıt ÇIRAKOĞLU Marmara Üniversitesi Tıp Fakültesi Tıbbi Biyoloji ve Genetik . Haydarpaşa, İstanbul-TURKEY Received: Abstract: We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM 3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found to be 10-8–10-9 M. Monoclonal antibodies did not cross react with the other steroid hormones. Key Words: Immunodiagnosis, hybrid cell, monoclonal antibody, steroid hormones, ELISA. Eş Zamanlı İmmünizasyonla Progesteron Estradiol ve Testosteron’a Özgü Monoklonal Antikorların Birlikte Üretilmesi Özet: Bu makalede karma immünizasyon tekniği ve hibridoma teknolojisi kullanılarak iki farklı steroid hormona özgün monoklonal antikorlar sentezleyen hibrit hücrelerinin geliştirilmesi rapor edilmektedir. Her biri sığır serum albuminine bağlı progesteron, estradiol ve testosteron’dan oluşan steroid hormon karışımı ile BALB/c fareler immünize edilmiştir. Bu fareler füzyon için kullanılmıştır. İki füzyon deneyinde, 645 kuyuda hibrit klon gözlenmiş bu klonlar içerisinde 2 tanesinin (MAM 3C2, MAM 3E3) progesteron ile reaksiyon verdiği bir tanesinin de ( MAM 7H7) estradiole özgü olduğu .

• Sinh học
• Hybrid cell
• Monoclonal antibody
• Steroid hormones
• Hybridoma technology
• Serum albumine
• Hybrid beamforming
• 5G millimeter wave multi cell networks
• Multi cell networks
• Multi cell wireless systems
• Mitigated via coordinated multipoint
• BMC Cancer
• Hybrid cell death
• K+ homeostasis
• Intracellular Ca2+
• Glioblastoma cells
• BMC Molecular and Cell Biology
• Gene amplification
• Double minutes
• Cell fusion
• Human rodent hybrid cells
• Genome Biology
• Single cell RNA seq
• Interactions between mesenchyme and epithelium
• Mesenchymal hybrid state
• Embryonic development
• Cancer stem cells
• Breast cancer
• Pathophysiological processes
• Several physiological
• Silver nanoparticles
• Silica hybrid materials
• Antibacterial activity
• Cell adhesion
• Tissue engineering
• Wound healing
• Hybrid complexes
• Hyaluronic acid
• Especially considering
• Injury site
• BMC Plant Biology
• Cell morphogenesis
• RNA interference
• RNA silencing
• Gene silencing
• Epidermal cell
• Hybrid proline rich protein
• Verticillium dahliae
• Cell wall protein
• Reactive oxygen species
• Virus induced gene silencing
• Phenylpropanoid metabolism
• Guaiacyl ratio
• Gene expression
• Eucalyptus urophylla
• Eucalyptus grandis hybrid
• Energy metabolism
• Pleomorphic sarcoma
• Highly rearranged genome
• Increased spreading capacities
• tài liệu hóa học
• tài liệu dầu khí
• giáo trình hóa học
• giáo trình dầu khí
• chuyên ngành dầu khí
• Hệ thống lái
• hệ thống đánh lửa
• hệ thống gầm
• chế hòa khí
• động cơ xăng
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Model Selection Tests
• Stem Cell
• Novel Pathogenesis
• Pathogenesis of Endometriosis
• Green Tea
• Tea for Endometriosis
• Harnessing opportunities
• Integrated approach
• Cooperation at multiple levels
• Endowment opportunities
• atmospheric resources
• realizing opportunities
• Report medicine
• traditional medicine
• scientific reports
• Antimicrobial
• cell structure
• genetics
• Water stress
• Relative water content
• Leaf water potential
• Epicuticular wax
• Cell membrane stability
• Chlorophyll stability index
• Hybrid peptide
• Humoral and cellular response
• Th1 deriving response
• B cell epitopes
• Cancer inhibitors
• Classification of cancer inhibitors
• Non inhibitors
• Active substructure
• Active functional groups
• Potency score
• Mammosphere formation capacity
• Colony formation capacity
• ALDH1 positive cancer cells