TAILIEUCHUNG - Fractionation of phenolic compounds from sonneratia apetala pneumatophores and their bioactivities
Sonneratia apetala pneumatophore samples were collected from Xuan Thuy National Park, and then 80% methanol extract was fractionated through Sephadex LH-20 column chromatography. After elution with 95% ethanol and 50% acetone, two phenolic fractions were obtained from the crude extract of of S. apetala pneumatophores. | TAP CHI SINH HOC 2017, 39(4): 451-456 Fractionation of phenolic compounds from Sonneratia apetala DOI: FRACTIONATION OF PHENOLIC COMPOUNDS FROM Sonneratia apetala PNEUMATOPHORES AND THEIR BIOACTIVITIES Nguyen Sao Mai, Dao Van Tan* Hanoi National University of Education, Hanoi ABSTRACT: Sonneratia apetala pneumatophore samples were collected from Xuan Thuy National Park, and then 80% methanol extract was fractionated through Sephadex LH-20 column chromatography. After elution with 95% ethanol and 50% acetone, two phenolic fractions were obtained from the crude extract of of S. apetala pneumatophores. The acetone fraction consisted of tannins having high antioxidant activity, times higher than that of ascorbic acid. This fraction also had higher activity of α-amylase inhibition (IC50 mg/mL) and R. sativus root growth inhibition (IC50 mg/mL) than did the ethanol fraction. In contrast, ethanol fraction consisted of low-molecular phenolic compounds having inhibitory effects on Samonella tiphimurium growth. Such antibacterial activity was not observed in the acetone extract. Keywords: Sonneratia apetala, tannin, low-molecular phenolic, antioxidant, α-amylase inhibition, root growth inhibition, anti-biotic activity Citation: Nguyen Sao Mai, Dao Van Tan, 2017. Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities. Tap chi Sinh hoc, 39(4): 451-456. DOI: . *Corresponding author: daotanvn@ Received 19 September 2017, accepted 12 December 2017 INTRODUCTION Recently mangrove plants are considered as a rich source of natural bioactive compounds. Mangrove is attractive primarily because it can survive and grow in extremely stressful environments, such as severe salinity, extreme tides, strong winds, high temperatures and muddy anaerobic soils. In addition to unique morphological features as the survival strategy, mangrove plants acquired various other
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