TAILIEUCHUNG - Genetic diversity among natural populations of keteleeria evelyniana mast. in Tay Nguyen of Viet nam using SSR markers

. Keteleeria evelyniana Mast. is a big softwood species with high economic values. Therefore, the number of individuals are rapidly decreasing due to rampant exploitation as well as its habitat loss and recently, the species is considered vulnerable in Viet Nam. In this study, we assessed the genetic variation among seventy K. evelyniana samples of three natural populations in Lam Dong, Dak Lak and Kon Tum using 16 microsatellite markers. The results showed that thirteen markers were polymorphic. | Vietnam Journal of Science and Technology 56 (3) (2018) 275-285 DOI: GENETIC DIVERSITY AMONG NATURAL POPULATIONS OF Keteleeria evelyniana Mast. IN TAY NGUYEN OF VIET NAM USING SSR MARKERS Tran Thi Lieu*, Vu Thi Thu Hien, Dinh Thi Phong Vietnam National Museum of Nature, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi * Email: tranthilieu@ Received: 16 May 2017; Accepted for publication: 16 March 2018 Abstract. Keteleeria evelyniana Mast. is a big softwood species with high economic values. Therefore, the number of individuals are rapidly decreasing due to rampant exploitation as well as its habitat loss and recently, the species is considered vulnerable in Viet Nam. In this study, we assessed the genetic variation among seventy K. evelyniana samples of three natural populations in Lam Dong, Dak Lak and Kon Tum using 16 microsatellite markers. The results showed that thirteen markers were polymorphic. A total 39 DNA fragments were amplified, among them, thirty – five were polymorphic (accounting for %). Among studied populations, the level of genetic diversity at Lam Dong (Na = ; Ne = ; Ap = ; I = ; Ho = and He = ) was the highest. Analysis of molecular variance (ANOVA) showed that the total level of molecular changes between populations was % and between individuals in the same population was %. Private alleles (Ap) and inbreeding values (Fis) of K. evelyniana species were founded of all three populations in Lam Dong, Dak Lak and Kon Tum ( and - ; and - ; and - , respectively). The gene flow (Nm) also occurred among the K. evelyniana populations with the average of Nm = . A dendrogram (UPGMA) constructed based on the similarity matrix of 70 K. evelyniana samples divided into two main groups with their genetic similarity coefficient ranged from % (Ke26 and Ke44) to 99 % (Ke23 and Ke25). The obtained results indicated the importance .

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