TAILIEUCHUNG - Significance of absorption spectra for the chemotaxonomic characterization of pigmented bacteria

A method for characterizing pigmented bacteria based on the numerical analysis of their carotenoid absorption spectra was examined. To achieve this, some non-phototrophic pigmented bacteria that were isolated from various environments and closely related types of species were used. | Turk J Biol 35 (2011) 167-175 © TÜBİTAK doi: Significance of absorption spectra for the chemotaxonomic characterization of pigmented bacteria Nurettin ŞAHİN Faculty of Education, Muğla University, 48170 Muğla - TURKEY Received: Abstract: A method for characterizing pigmented bacteria based on the numerical analysis of their carotenoid absorption spectra was examined. To achieve this, some non-phototrophic pigmented bacteria that were isolated from various environments and closely related types of species were used. Bacterial carotenoid pigments were extracted with an acetone:methanol (7:2) mixture and the absorption spectra were determined. Data were normalized, and their first derivatives were analyzed using Ward clustering algorithms. Based on the spectral data, isolates were separated into 7 groups. Characterization based on derivative pigment spectral data showed good agreement statistically with biochemical characterization results. The Mantel test indicated that the dissimilarity matrices of phenotypic tests and the pigment spectral data were correlated (P < ). Absorption spectra can simplify and accelerate the identification and characterization of pigmented bacteria, when used with other distinctive biochemical tests. This method might be useful for determination of the microbial community structure in a variety of ecological studies. Key words: Bacterial carotenoids, chemotaxonomy, pigment absorption spectra Pigmentli bakterilerin kemotaksonomik karakterizasyonu için absorbsiyon spektrumlarının önemi Özet: Bu çalışmada pigmentli bakterilerin karakterizasyonu için karotenoid absorpsiyon spektrumlarının numerik analizine dayalı bir metod tartışılmıştır. Bu amaçla, çeşitli kaynaklardan fototrofik olmayan, pigmentli bakteriler izole edilmiş ve yakın tip türler kullanılmıştır. Bakteriyal karotenoid pigmentler aseton-methanol (7:2) karışımı ile ekstrakte edilmiş ve absorpsiyon spektrumları belirlenmiştir. Elde edilen .

• Sinh học
• Bacterial carotenoids
• Microbial community structure
• Pigment absorption spectra
• Tryptic soy agar
• 16S rRNA sequencing
• MDR salmonella
• 16S rRNA gene
• 23S rRNA gene
• RNA sequencing method
• Multidrug resistant salmonella serovars isolated
• Analyzing 16s rRNA sequences
• Antigenic genes
• Leptospirosis recombinant vaccine
• 16S rRNA gene sequencing
• In silico prediction of potential antigenic epitopes
• BMC Genomics
• Low biomass
• Airway microbiome
• Laboratory protocols
• High throughput sequencing
• 16S ribosomal RNA
• Luận văn Thạc sĩ
• Luận văn Khoa học
• Vi sinh vật liên kết với hải miên
• Kỹ thuật 16s rRNA Miseq Sequencing
• Nghiên cứu metagenomics
• BMC Bioinformatics
• 16S rRNA
• Paired end sequencing
• Quality filtering
• Pre processing
• Extremely low birth weight infants
• Shotgun sequencing
• DNA extraction
• Heavy metal resistant bacteria
• Copper bioremoval
• Lysogeny broth
• DNA sequencing
• BMC Veterinary Research
• Fecal metabolites
• Gut microbiota
• Panthera pardus japonensis
• Habitat fragmentation
• Primer design
• Multi objective optimization
• Targeted amplicon sequencing
• BMC Musculoskeletal Disorders
• Prosthesis related infections
• Infected joint arthroplasty treatment
• Unidentified microorganisms
• Error correction
• 16S rRNA amplicon sequencing
• 454 pyrosequencing
• Sequencing technologies
• Illumina miseq based sequencing
• Bacterial community
• Vietnamese ginseng cultivated soil
• Obtained 16S rRNA data
• Bacterial population in soil
• Fungal defense
• RNA sequencing
• Pore forming toxins
• Bacterial pore forming toxins
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Halo blight
• Pseudomonas syringae (savastoni) pv phaseolicola
• Seeds grown in Rajasthan state
• Molecular identification of Pseudomonas syringae
• Mycobacterium tuberculosis
• RT PCR
• Axis axis
• Mycobacterium tuberculosis infection
• Enterococcus faecalis
• Multiple antibiotic resistance
• 16s rRNA amplification and sequencing
• Diseased tilapia
• Antibiotic resistance of enterococcus faecalis
• Bacteria detection
• NGS analysis
• Microbial genomes
• Next generation sequencing data
• Homology based methods
• In silico
• Microbial diversity
• Evolutionary history
• Iron nanoparticles synthesizing bacteria contributing
• Endophytic bacteria
• Bacillus sp
• Endophytic bacteria from tomato
• Molecular characterization of endophytic bacteria
• Whole genome sequencing
• Functional profiles of microbiome
• Phylogenetically related genomes
• Functional reconstruction
• Apical periodontitis
• Root canal treatment
• Healthy control
• BMC Pulmonary medicine
• Next generation sequencing
• Obstructive lung diseaseases
• Microbiota identified
• Protected specimen brushes