TAILIEUCHUNG - Utilization of cytochrome oxidase I in Cephus pygmeus (L.) (Hymenoptera: Cephidae)

The mitochondrial cytochrome oxidase I (COI) gene region of Cephus pygmeus (L.) (Hymenoptera: Cephidae) was characterized at the nucleotide and amino acid levels. The partial sequence (687 bp) of the gene was determined and compared with other cephids and insect species at both the nucleic acid and amino acid sequence levels. | E. M. KORKMAZ, M. BUDAK, H. H. BAŞIBÜYÜK Turk J Biol 35 (2011) 713-726 © TÜBİTAK doi: Utilization of cytochrome oxidase I in Cephus pygmeus (L.) (Hymenoptera: Cephidae) Ertan Mahir KORKMAZ1, Mahir BUDAK2, Hasan Hüseyin BAŞIBÜYÜK2 1 Department of Molecular Biology and Genetics, Faculty of Science, Cumhuriyet University, 58140 Sivas - TURKEY 2 Department of Biology, Faculty of Science, Cumhuriyet University, 58140 Sivas - TURKEY Received: Abstract: The mitochondrial cytochrome oxidase I (COI) gene region of Cephus pygmeus (L.) (Hymenoptera: Cephidae) was characterized at the nucleotide and amino acid levels. The partial sequence (687 bp) of the gene was determined and compared with other cephids and insect species at both the nucleic acid and amino acid sequence levels. The examined fragment was placed at an equal distance to both ends of the gene and consisted of 13 structural regions. The 5’ end of the partial COI gene was more informative than the 3’ end at the intraspecific level. Pairwise sequence divergences ranged from to at the intraspecific level, while the rate varied from to in comparison with other Cephus species. The percentage of nucleotide composition at each codon position and the rate of nucleotide substitutions were variable and displayed a bias toward A + T. A strong bias of transitional substitutions was observed at the intraspecific level, but there was a lower rate at the interspecific level. The codon usage patterns and the extent of the substitutions at synonymous codons were also investigated at the amino acid level. These results suggest that the characterized region is informative for estimating both intra- and interspecific relationships due to its possession of both completely conserved and variable regions. Key words: Cephidae, COI, substitution bias, codon usage Cephus pygmeus (L.) (Hymenoptera: Cephidae)’da sitokrom oksidaz I’in kullanımı Özet: Cephus pygmeus (L.) .

• Sinh học
• Cytochrome oxidase I
• Substitution bias
• Codon usage
• Cephus pygmeus L
• Amino acid
• Genetic distance
• Phylogenetic relationship based
• Mitochondrial Cytochrome Oxidase I
• Brinjal shoot
• Fruit borer
• Journal of Zoology
• Phylogenetic analysis
• Mitochondrial cytochrome oxidase I gene
• Bayesian inference
• Maximum likelihood
• Cá dìa công Siganus guttatus
• COI mtDNA
• Đa dạng di truyền quần thể cá dìa công
• Biển Cù Lao chàm
• Kiểu gen Cytochrome Oxidase I
• Orthochirus sp
• Genetic diversity
• Cytochrome c oxidase
• Mitochondrial 16S rRNA gene
• Androctonus crassicauda
• Chi cá nheo Silurus
• DNA ty thể
• Gen mã hóa cytochrome C oxidase subunit I
• Phân loại phân tử
• Đoạn gen COI
• Kỹ thuật PCR
• BMC Plant Biology
• Pitcher plant
• DNA barcoding
• Nepenthes genus
• Tyrrhenian Sea
• Cryptic diversity
• Rare species
• Cytochrome c oxidase subunit I (COI)
• Zaprionus tuberculatus
• Molecular identification
• Cytochrome oxidase I (COI)
• Ceratitis capitata
• Loris lydekkerianus
• Loris tardigradus
• mtDNA CO1 barcoding
• Phylogenetic relationship
• Cytochrome oxidase I COI
• Black cherry aphid
• Nucleotide diversity
• Cytochrome c oxidase subunit I
• 28S rRNA
• DNA taxonomy
• Eriophyoid mites
• Mã vạch DNA
• Loài tu hài
• Lutraria rhychaena
• Trình tự vùng gene COI