TAILIEUCHUNG - Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water

The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase (PG), pectin lyase (PNL), xylanase, and cellulase, of some pectinolytic bacteria were studied. | B. DAS, A. CHAKRABORTY, S. GHOSH, K. CHAKRABARTI Turk J Biol 35 (2011) 671-678 © TÜBİTAK doi: Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water Biswapriya DAS1, Ashis CHAKRABORTY2, Sagarmoy GHOSH3, Kalyan CHAKRABARTI1 1 Department of Agricultural Chemistry and Soil Science. Institute of Agricultural Science, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019 - INDIA 2 3 Department of Agronomy, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, 741252 - INDIA Department Of Microbiology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019 - INDIA Received: Abstract: The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase (PG), pectin lyase (PNL), xylanase, and cellulase, of some pectinolytic bacteria were studied. The pectinolytic bacterial strains were isolated from the jute retting water bodies of 2 important jute growing areas of North 24 Parganas, West Bengal, India. The highest PG ( to IU/g cell wet weight) and PNL ( to U/mL) activities occurred at the alkaline pH of and , respectively. Maximum xylanase activity ( to μmol/mL/min) occurred at pH . An acidic pH of favored the cellulase activity (0 to μmol/mL/ min). All the enzymes were highly substrate specific and inducible in nature. The highest PG and PNL activities were observed in the presence of pectin, whereas the highest xylanase and cellulase activities were detected in the presence of xylan and cellulose, respectively, as the sole carbon source in the media. Key words: Pectinolytic bacteria, polygalacturonase, pectin lyase, xylanase, cellulase Introduction Jute (Corchorus sp.) is one of the most versatile bast fibers, occupying second position among the natural industrial fibers of the world and .

• Sinh học
• Pectinolytic bacteria
• Pectin lyase
• Namely polygalacturonase
• Jute Corchorus sp
• K cetyltrimethylammonium bromide
• BMC Genomics
• Soft rot
• Erwinia chrysanthemi
• Next generation sequencing
• Whole genome sequencing
• Pacific biosciences
• Average nucleotide identity