TAILIEUCHUNG - Acid proteinase enzyme activity in Candida albicans strains: A comparison of spectrophotometry and plate methods

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. | S. AKÇAĞLAR, B. ENER, O. TÖRE Turk J Biol 35 (2011) 559-567 © TÜBİTAK doi: Acid proteinase enzyme activity in Candida albicans strains: a comparison of spectrophotometry and plate methods Sevim AKÇAĞLAR*, Beyza ENER, Okan TÖRE Uludağ University, Faculty of Medicine, Department of Microbiology and Infectious Diseases, 16059 Bursa - TURKEY Received: Abstract: In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > ). Key words: Candida albicans, secreted aspartyl proteinase (SAP) activity, virulence Candida albicans suşlarında asit proteinaz enzim aktivitesi: spektrofotometri ve petri metodlarının karşılaştırılması Özet: Son yıllarda bütün dünyada fungal enfeksiyon olgularında artış gözlenmektedir. Patojenik funguslarla ilgili araştırmalar artmış olmasına rağmen, hala virulansla ilgili çalışmalara ihtiyaç vardır. Bu çalışmada kandan izole edilen Candida albicans suşlarında in vitro virulans faktörlerinin araştırılması amaçlanmıştır. Steril vücut sıvılarından izole edilen 30 C. albicans (15’i kolonizasyon, 15’i enfeksiyon) suşlarının proteinaz enzim aktivitesi spektrofotometrik ve petri plak yöntemi ile değerlendirilmiştir. İki yöntem .

• Sinh học
• Candida albicans
• Secreted aspartyl proteinase activity
• Acid proteinase
• Enzyme activity
• Bloodstream identified
• Kỹ thuật seminested PCR
• Nấm Candida albicans
• Chủng nấm Candida albicans phân lập Việt Nam
• Phương pháp xét nghiệm chẩn đoán nấm
• Trình tự đoạn gen 410 bp của nấm Candida albicans
• Chế tạo nano berberin
• Kháng sinh thực vật
• Kháng nấm Candida albicans
• Trị bệnh do nấm Candida albicans gây ra
• Ức chế phát triển của nấm Candida albicans
• Antifungal activity
• Aspergillus fumigatus
• Essential oil
• Pathogenic fungi Candida albicans
• Essential oils from some plants
• Viêm âm đạo do nấm
• Kháng thuốc chống nấm của Candida albicans
• Kháng thuốc 5 flucytosine
• Thuốc chống nấm nhóm echinocandin
• Nghiên cứu y học
• Nhiễm nấm Candida albicans
• Định danh nấm Candida albicans
• Nhiễm trùng máu
• Sinh bài tử dày
• Tạp chí y học
• Mức độ nhạy cảm
• Candida spp với fluconazole
• Định danh các chủng candida
• Candida albicans và candida non albicans
• Immuno compromised
• Candida non albicans
• Tertiary care hospital
• Candida species amongst various clinical samples
• Native polyacrylamide gel electrophoresis
• Electrophoretic analysis
• Candida albicans Isolates
• C
• albicans strains
• Sodium dodecyl sulfate
• Germ tube production
• Retrospective study
• Hi chrome candida agar
• Various clinical samples
• Non – albicans Antifungal susceptibility
• Tertiary care teaching hospital
• Candida isolates from clinical samples
• Non albicans Candida
• Antifungal susceptibility
• Hospitalised patients
• CHROM agar
• Antifungal susceptibility testing
• Various Candida species
• Candida and its comparison
• Antifungal susceptibility profile
• Steroids or other immunosuppressive agents
• Carbohydrate polymers
• Candida spp
• Molecular weight
• Candida tropicalis
• Candida parapsilosis sensu stricto
• Diabetes mellitus
• Candida species
• Diabetes mellitus patient
• Scientific reports
• biochemistry
• molecular Biology
• oxygen species
• tumor cells
• Germ tube
• Rapid germ tube production
• Glucose Glycine medium
• Hyphal form cell
• Kháng candida albicans
• Vi nấm nội sinh
• Nuôi cấy hoạt tính kháng
• chất kháng khuẩn
• Bảng mỏng sắc ký bằng
• Kỹ thuật hiện hình sinh học
• Genital infection
• Female rat model
• In vitro antifungal
• Biologic activity
• Immunosuppressive activity
• Bài viết về y học
• Dị hoàng thang
• Vị hoàng bá trên in vitro
• Tạo vòng kháng nấm
• Alcohol dehydrogenase
• Moonlighting proteins
• Macrophage differentiation
• ERK pathway
• Microbial growth
• Parenteral nutrition
• Blood stream infection
• Peripheral parenteral nutrition
• Pathogen isolated
• Patients attending tertiary care centre
• Candiduria cases commonly observed
• Bệnh viện Da liễu Trung ương
• Bệnh nhân viêm âm hộ
• Viêm âm đạo
• Bệnh nhiễm trùng