TAILIEUCHUNG - Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA
In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 18-24 © TÜBİTAK doi: Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on rDNA Melike ÇEBİ KILIÇOĞLU*, İbrahim ÖZKOÇ Department of Biology, Faculty of Arts and Science, Ondokuz Mayıs University, Samsun, Turkey Received: Accepted: Published Online: Printed: Abstract: In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups (especially AG-4 HG-I). This study represents the first reported use of rDNA-ITS sequence data to examine AG-4 HG-I and HG-II in P. vulgaris from Turkey. The results also suggest that the Giresun isolates are closely related to isolates obtained from Italy. Key words: rDNA-ITS phylogeny, Rhizoctonia solani, AG-4, Phaseolus vulgaris, common bean 1. Introduction Rhizoctonia solani Kühn [teleomorph: Thanatephorus cucumeris (Frank) Donk] is a soil-borne fungus that causes economically damaging diseases in many crop species (1). The species consists of many related but genetically isolated groups (2). Based on hyphal anastomosis reaction, R. solani can be divided into 13 anastomosis groups (AGs) (3). Some AGs of R. solani have been further divided into subgroups based on cultural morphology, host range, virulence,
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