TAILIEUCHUNG - A study on the lipase-catalayzed esterification in organic solvent

This study was undertaken to investigate effect of molecular sieves on the esterification reaction between lauric acid and geraniol in isooctane catalyzed by immobilized lipase in a batch and continuous-loop packed-bed reactor. Molecular sieves were used to reduce the inhibitive effect of water formed during the reaction by removing it as it formed. | Tr. J. of Agriculture and Forestry 22 (1998) 573-578 © TÜBİTAK A Study on the Lipase-Catalayzed Esterification in Organic Solvent Mustafa Ümit ÜNAL Çukurova Üniversitesi, Ziraat Fakültesi, Gıda Mühendisliği Bölümü, Adana-TURKEY Received: Abstract: This study was undertaken to investigate effect of molecular sieves on the esterification reaction between lauric acid and geraniol in isooctane catalyzed by immobilized lipase in a batch and continuous-loop packed-bed reactor. Molecular sieves were used to reduce the inhibitive effect of water formed during the reaction by removing it as it formed. Results in a batch reactor showed that, for the molecular sieves to have a pronounced effect on the performance of the enzyme, a ratio of at least 2:1 with respect to the weight of the immobilized enzyme must be used. It was also found that the ability of molecular sieves to reduce inhibition by water is more noticeable when working in a packed-bed reactor. The physical contact between the molecular sieves and enzymes is of utmost importance for the adsorption of water since water is markedly insoluble in the organic solvent. Organik Çözgende Lipaz Enzimince Katalizlenen Esterleşme Reaksiyonu Üzerinde Bir Araştırma Özet: Bu çalışmada, moleküler süzgeçlerin laurik asit ve geraniol arasındaki esterleşme reaksiyonu üzerine etkisi araştırılmıştır. Reaktör olarak kesikli ve sürekli paketlenmiş-yatak reaktörleri kullanılmıştır. Reaksiyon izooktan çözgeni içerisinde gerçekleştirilmiş ve tutuklanmış lipaz enzimince katalizlenmiştir. Moleküler süzgeçler, reaksiyon sırasında oluşan suyu uzaklaştırarak suyun reaksiyon üzerine olan inhibe edici etkisini azaltmak için kullanılmıştır. Kesikli reaktörlerde yapılan deneylerden elde edilen sonuçlar, moleküler süzgeçlerin enzimlerin performansı üzerine belirgin bir etkiye sahip olabilmesi için, moleküler süzgeç enzim oranının en az 2:1 (ağırlık üzerinden) olması gerektiğini göstermiştir. Ayrıca, moleküler süzgeçlerin suyun inhibe

• Nông nghiệp
• Lipase catalayzed esterification
• Organic solvent
• Lauric acid
• Continuous loop packed bed reactor
• Enzymatic catalysis
• Scientific reports
• scientific research
• molecular
• Enzymatic
• snake venom
• genetics
• Carbohydrate polymers
• Synthetic cellulose
• Ionic liquid
• Chain self assembly
• Phosphorylase bio catalysis