TAILIEUCHUNG - Báo cáo khao học: Assessment of Tamarindus indica Extracts for Antibacterial Activity

Ethanolic and aqueous (hot and cold) extracts of the fruit pulp, stem bark and leaves of Tamarindus indica were evaluated for antibacterial activity, in vitro, against 13 Gram negative and 5 Gram positive bacterial strains using agar well diffusion and macro broth dilution techniques, simultaneously. | Int. J. Mol. Sci. 2011 12 6385-6396 doi ijms12106385 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www. j ournal ijms Article Assessment of Tamarindus indica Extracts for Antibacterial Activity Uchechukwu U. Nwodo 1 J Grace E. Obiiyeke 2 Vincent N. Chigor 1 and Anthony I. Okoh 1 1 Applied and Environmental Microbiology Research Group AEMREG Department of Biochemistry and Microbiology University of Fort Hare Private Bag X1314 Alice 5700 South Africa E-Mails vchigor@ . aokoh@ . 2 Department of Botany Delta State University Abraka 330106 Delta State Nigeria E-Mail ekygee@ Author to whom correspondence should be addressed E-Mail unwodo@ or uchenwodo@ Tel. 27-786273279. Received 30 August 2011 in revised form 19 September 2011 Accepted 22 September 2011 Published 26 September 2011 Abstract Ethanolic and aqueous hot and cold extracts of the fruit pulp stem bark and leaves of Tamarindus indica were evaluated for antibacterial activity in vitro against 13 Gram negative and 5 Gram positive bacterial strains using agar well diffusion and macro broth dilution techniques simultaneously. The fruit pulp extracts exhibited a wide spectrum of activity the cold water extract against of the test bacterial strains and the hot water and ethanolic extracts against and respectively. In contrast the cold water extract of the leaves and stem bark each was active against while the ethanolic extract of each was active against 75 of the test strains. The minimum inhibitory concentrations MIC ranged from mg mL against Bacillus subtilis ATCC 6051 to mg mL against Escherichia coli ATCC 11775 and the minimum bactericidal concentration MBC ranged from 125 mg mL against Pseudomonas aeruginosa ATCC 10145 to 250 mg mL against Bacillus subtilis ATCC 6051. Keywords Tamarind Tamarindus indica extracts antibacterial activity Int. J. Mol. Sci. 2011 12 6386 1. Introduction All .

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