TAILIEUCHUNG - Characterisation and homology modelling of finfish NF-KAPPA B inhibitor alpha using in silicoanalysis

NFκB plays an important role in the immune system in all organisms. In this study, physicochemical properties and modelling of finfish NFκBIα protein was analysed using in silico approach. Finfish species: guppy (Poecilia reticulate), Bicolor damselfish (Stegastes partitus), Channel catfish (Ictalurus punctatus), Coelacanth (Latimeria chalumnae), and Australian ghostshark (Callorhinchus milii) were used in this study. | J. Sci. Devel. 2015 Vol. 13 No. 2 216-225 Tạp chí Khoa học và Phát triển 2015 tập 13 số 2 216-225 CHARACTERISATION AND HOMOLOGY MODELLING OF FINFISH NF-KAPPA B INHIBITOR ALPHA USING N SILICO ANALYSIS Tran Ngoc Tuan1 WangWei-Min1 and Pham Minh Duc2 1College of Fisheries Huazhong Agricultural University China 2College of Aquaculture and Fisheries Can Tho University Viet Nam Email tranntts@ Received date Accepted date ABSTRACT NFkB plays an important role in the immune system in all organisms. In this study physicochemical properties and modelling of finfish NFkBIo protein was analysed using in silico approach. Finfish species guppy Poecilia reticulate Bicolor damselfish Stegastes partitus Channel catfish Ictalurus punctatus Coelacanth Latimeria chalumnae and Australian ghostshark Callorhinchus milii were used in this study. Physicochemical characteristics molecular weight Mol. Wt. - theoretical isoelectric point pl - extinction coefficient EC 17920- 37650 17420 - 36900 aliphatic index AI - instability index II - total number of negatively charged residues -R 47 - 55 and positively charged residues R 19 - 29 and grand average of hydropathicity GRAVY - were obtained. The results showed that except NFkBIo from P. reticulate all were defined as soluble proteins. Possible pairing and pattern of cysteine residues were found in all protein sequences and the most probable pattern of pairs of cysteine. Secondary structure analysis revealed approximately equal parts of random coils and helices followed by strands. Three dimensional homology modelling for NFkBIo from finfish was performed and evaluated as credible models based on PROCHECK s Ramachandran plot ProQ and ProSA analysis. Keywords in silico finfish NfKBIa. Định tính chất và mô hình tương đồng yếu tố nhân kappa B alpha của cá sử dụng phương pháp mô phỏng máy tính TÓM TẮT Yếu tố nhân kappa B NFkB đóng vai trò

• Sinh học
• Finfish NF KAPPA B
• Modelling of finfish NF KAPPA B
• Định tính chất
• Phương pháp mô phỏng máy tính
• Yếu tố nhân kappa B alpha
• NFκB plays