TAILIEUCHUNG - Development of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis

In the present study intestinal delivery systems resistant to gastric juice, loaded with the probiotic bacteria Lactobacillus acidophilus LA14 and Bifidobacterium lactis BI07, were produced by the polyelectrolyte complexation. First, beads were prepared by the traditional extrusion method and nine formulations were developed using alginate as main carrier and the biopolymer, xanthan gum, as hydrophilic retardant polymer or the cellulose derivative, cellulose acetate phthalateDevelopment of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis. | European Journal of Pharmaceutical Sciences 40 2010 359-366 Contents lists available at ScienceDirect if European Journal of Pharmaceutical Sciences ELSEVIER journal homepage locate ejps Development of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis Beatrice Albertinia Beatrice Vitalib Nadia Passerinia Federica Crucianib Marcello Di Sabatinoa Lorenzo Rodrigueza Patrizia Brigidib a Department of Pharmaceutical Sciences University of Bologna Via San Donato 19 2 40127 Bologna Italy b Department of Pharmaceutical Sciences University of Bologna Via Belmeloro 6 40126 Bologna Italy ARTICLE INFO ABSTRACT Article history Received 28 January 2010 Received in revised form 3 March 2010 Accepted 19 April 2010 Available online 24 April 2010 Keywords Probiotics Alginate microcapsules Cell microencapsulation Viability Gastric protection Stability In the present study intestinal delivery systems resistant to gastric juice loaded with the probiotic bacteria Lactobacillus acidophilus LA14 and Bifidobacterium lactis BI07 were produced by the polyelectrolyte complexation. First beads were prepared by the traditional extrusion method and nine formulations were developed using alginate as main carrier and the biopolymer xanthan gum XG as hydrophilic retardant polymer or the cellulose derivative cellulose acetate phthalate CAP as gastro-resistant polymer. The results showed that the incorporation of the w v of XG or the 1 w v of CAP within the 3 w v of alginate solution increased the survival of the probiotic bacteria in acid conditions from 63 of the freeze-dried bacteria up to 76 . Subsequently these formula was used to prepare smaller microcapsules by means of an atomization device. Despite of the high viscosity of the biomass suspension the spraying system produced spherical and non-aggregated microcapsules able to survive in harsh condition better than beads the survival of the probiotic bacteria after acid

• Vật lý
• Development of microparticulate
• Systems for intestinal delivery
• Lactobacillus acidophilus
• Bifidobacterium lactis
• Materials and methods
• Bacterial strains and culture conditions
• Bacterial strains
• Culture conditions
• Bacterial strain and culture conditions
• Grape pomace extract
• Statistical analysis
• Ligninolytic enzymes
• Lignin peroxidase
• manganese peroxidase
• Five bacterial strains
• Culture conditions on ligninolytic enzyme
• Activity of five bacterial strains