TAILIEUCHUNG - The role of chitosan in protection of soybean from sudden death syndrome caused by Fusarium solani f. sp. glycines

The role of chitosan in protection of soybean from sudden death syndrome caused by Fusarium solani f. sp. glycines has many contents: introduction, methods, fungal culture and growth, evaluation of the role of chitosan in protection of soybean from SDS development, chitinase activity assay in soybean leaves, statistical analysis, eVects of chitosan as a natural antifungal agenton inhibition of the radial and submerged growth ofF. solani f. sp. glycines,. | ELSEVIER Available online ScienceDirect Bioresource Technology 98 2007 1353-1358 bomiixT TKtlliOLOQT The role of chitosan in protection of soybean from sudden death syndrome caused by Fusarium solani f. sp. glycines Benjaphorn Prapagdee a Kanignun Kotchadat a Acharaporn Kumsopa a Niphon Visarathanonth b a Faculty of Environment and Resource Studies Mahidol University Salaya Nakhon Pathom 73170 Thailand b Department of Plant Pathology Faculty of Agriculture Kasetsart University Bang Khen Bangkok 10220 Thailand Received 12 September 2005 received in revised form 18 May 2006 accepted 21 May 2006 Available online 7 July 2006 Abstract The in vitro antifungal properties of chitosan and its role in protection of soybean from a sudden death syndrome SDS were evaluated. Chitosan inhibited the radial and submerged growth of F. solani f. sp. glycines with a marked effect at concentrations up to 1 mg ml indicating antifungal property and at 3 mg ml was able to delay SDS symptoms expression on soybean leaves for over three days after fungal inoculation when applied preventively. Chitosan was able to induce the level of chitinase activity in soybean resulting in the retardation of SDS development in soybean leaves. However the SDS symptoms gradually appeared and were associated with the reduction of chitinase activity level after five days of infection period. These results suggested the role of chitosan in partially protecting soybeans from F. solani f. sp. glycines infection. 2006 Elsevier Ltd. All rights reserved. Keywords Fusarium solani Chitosan Sudden death syndrome Soybean 1. Introduction Sudden death syndrome SDS caused by the soil-borne fungus F. solani f. sp. glycines is an economically harmful disease of soybean Rupe 1989 . SDS causes rapid defoliation of soybean resulting in reducing both the quality and quantity of soybean product Roy et al. 1989 Rupe 1989 . The development of SDS is favored by cool and wet rhizo-spheric conditions through .

• Nông nghiệp
• The role of chitosan in protection
• Fungal culture and growth
• Sudden death syndrome caused
• Chitinase activity assay in soybean
• The level of chitinase activity