TAILIEUCHUNG - Báo cáo hóa học: " Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí hóa hoc quốc tế đề tài : Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer | Selim et al. Nanoscale Research Letters 2011 6 528 http content 6 1 528 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Reduced cytotoxicity of insulin-immobilized CdS quantum dots using PEG as a spacer KM Kamruzzaman Selim1 Zhi-Cai Xing1 Moon-Jeong Choi2 Yongmin Chang3 Haiqing Guo4 and Inn-Kyu Kang1 Abstract Cytotoxicity is a severe problem for cadmium sulfide nanoparticles CSNPs in biological systems. In this study mercaptoacetic acid-coated CSNPs typical semiconductor Q-dots were synthesized in aqueous medium by the arrested precipitation method. Then amino-terminated polyethylene glycol PEG was conjugated to the surface of CSNPs PCSNPs in order to introduce amino groups to the surface. Finally insulin was immobilized on the surface of PCSNPs ICSNPs to reduce cytotoxicity as well as to enhance cell compatibility. The presence of insulin on the surface of ICSNPs was confirmed by observing infrared absorptions of amide I and II. The mean diameter of ICSNPs as determined by dynamic light scattering was about 38 nm. Human fibroblasts were cultured in the absence and presence of cadmium sulfide nanoparticles to evaluate cytotoxicity and cell compatibility. The results showed that the cytotoxicity of insulin-immobilized cadmium sulfide nanoparticles was significantly suppressed by usage of PEG as a spacer. In addition cell proliferation was highly facilitated by the addition of ICSNPs. The ICSNPs used in this study will be potentials to be used in bio-imaging applications. Keywords nanoparticles immobilization polyethylene glycol insulin cytotoxicity Introduction Recently quantum dots CdS CdSe ZnS CdTe etc. Q-dots have attracted tremendous interest as luminescent probes in biological and medical researches due to their unique optical and chemical properties 1 . Compared with traditional dyes and fluorescent proteins used as imaging probes Q-dots have several advantages such as tunable emission from visible to .

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