TAILIEUCHUNG - Báo cáo y học: " The inflammatory response seen when human omental adipose tissue explants are incubated in primary culture is not dependent upon albumin and is primarily in the nonfat cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The inflammatory response seen when human omental adipose tissue explants are incubated in primary culture is not dependent upon albumin and is primarily in the nonfat cells. | Fain et al. Journal of Inflammation 2010 7 4 http content 7 1 4 JOURNAL OF INFLAMMATION RESEARCH Open Access The inflammatory response seen when human omental adipose tissue explants are incubated in primary culture is not dependent upon albumin and is primarily in the nonfat cells John N Fain1 Paramjeet Cheema1 David S Tichansky2 Atul K Madan2 Abstract Background The present studies were designed to investigate the changes in gene expression during in vitro incubation of human visceral omental adipose tissue explants as well as fat cells and nonfat cells derived from omental fat. Methods Adipose tissue was obtained from extremely obese women undergoing bariatric surgery. Explants of the tissue as well as fat cells and the nonfat cells derived by digestion with collagenase were incubated for 20 minutes to 48 h. The expression of interleukin 1b IL-1b tumor necrosis factor a TNFa interleukin 8 IL-8 NFKB1p50 subunit hypoxia-inducible factor 1a HIF1a omentin intelectin and 11b-hydroxysteroid dehydrogenase 1 11b-HSD1 mRNA were measured by qPCR as well as the release of IL-8 and TNFa. Results There was an inflammatory response at 2 h in explants of omental adipose tissue that was reduced but not abolished in the absence of albumin from the incubation buffer for IL-8 IL-1b and TNFa. There was also an inflammatory response with regard to upregulation of HIF1a and NFkBI gene expression that was unaffected whether albumin was present or absent from the medium. In the nonfat cells derived by a 2 h collagenase digestion of omental fat there was an inflammatory response comparable but not greater than that seen in tissue. The exception was HIF1a where the marked increase in gene expression was primarily seen in intact tissue. The inflammatory response was not seen with respect to omentin intelectin. Over a subsequent 48 h incubation there was a marked increase in IL-8 mRNA expression and IL-8 release in adipose tissue explants that was also seen to

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