TAILIEUCHUNG - Báo cáo sinh học: "Alterations in the transcriptome and antibiotic susceptibility of Staphylococcus aureus grown in the presence of diclofenac"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: Alterations in the transcriptome and antibiotic susceptibility of Staphylococcus aureus grown in the presence of diclofenac. | Riordan et al. Annals of Clinical Microbiology and Antimicrobials 2011 10 30 http content 10 1 30 ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS RESEARCH Open Access Alterations in the transcriptome and antibiotic susceptibility of Staphylococcus aureus grown in the presence of diclofenac James T Riordan1 JoAnne M Dupre2 Stephanie A Cantore-Matyi2 Atul Kumar-Singh3 Yang Song3 c I . s r 7 i i 2 c I I I r 2 l I c I I kl sl1 ii r i l I z i M n I v 4 5 h ỉ r KỲ cs I CI r rì4Drì l A kr 3 -s Shahrear Zaman Sonia Horan Nada S Helal Vijayaraj Nagarajan Mohamed O Elasri Brian J Wilkinson and John E Gustafson2 Abstract Background Diclofenac is a non-steroidal anti-inflammatory drug NSAID which has been shown to increase the susceptibility of various bacteria to antimicrobials and demonstrated to have broad antimicrobial activity. This study describes transcriptome alterations in S. aureus strain COL grown with diclofenac and characterizes the effects of this NSAID on antibiotic susceptibility in laboratory clinical and diclofenac reduced-susceptibility DcRS S. aureus strains. Methods Transcriptional alterations in response to growth with diclofenac were measured using S. aureus gene expression microarrays and quantitative real-time PCR. Antimicrobial susceptibility was determined by agar diffusion MICs and gradient plate analysis. Ciprofloxacin accumulation was measured by fluorescence spectrophotometry. Results Growth of S. aureus strain COL with 80 pg ml X MIC of diclofenac resulted in the significant alteration by 2-fold of 458 genes. These represented genes encoding proteins for transport and binding protein and DNA synthesis and the cell envelope. Notable alterations included the strong down-regulation of antimicrobial efflux pumps including mepRAB and a putative emrAB qacA-family pump. Diclofenac up-regulated sigB ơB encoding an alternative sigma factor which has been shown to be important for antimicrobial resistance. .

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