TAILIEUCHUNG - Báo cáo sinh học: ""Hook"-calibration of GeneChip-microarrays: Theory and algorithm"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: "Hook"-calibration of GeneChip-microarrays: Theory and algorithm. | Algorithms for Molecular Biology BioMed Central Research Hook -calibration of GeneChip-microarrays Theory and algorithm Hans Binder 1 and Stephan Preibisch2 Open Access Address 1Interdisciplinary Centre for Bioinformatics University of Leipzig D-04107 Leipzig Germany. and 2Max-Planck-Institute for Molecular Cell Biology and Genetics D-01307 Dresden Germany. Email Hans Binder - binder@ Stephan Preibisch - preibisch@ Corresponding author Published 29 August 2008 Received 27 May 2008 Algorithms for Molecular Biology 2008 3 12 doi 1748-7188-3-12 Accepted 29 August 2008 This article is available from http content 3 1 12 2008 Binder and Preibisch licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The improvement of microarray calibration methods is an essential prerequisite for quantitative expression analysis. This issue requires the formulation of an appropriate model describing the basic relationship between the probe intensity and the specific transcript concentration in a complex environment of competing interactions the estimation of the magnitude these effects and their correction using the intensity information of a given chip and finally the development of practicable algorithms which judge the quality of a particular hybridization and estimate the expression degree from the intensity values. Results We present the so-called hook-calibration method which co-processes the log-difference delta and -sum sigma of the perfect match PM and mismatch MM probe-intensities. The MM probes are utilized as an internal reference which is subjected to the same hybridization law as the PM however with modified characteristics. After sequence-specific affinity correction

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