TAILIEUCHUNG - Báo cáo khoa học: Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus

Fish produce mucus substances as a defensive outer barrier against envi-ronmental xenobiotics and predators. Recently, we found a bioactive pro-tein in the mucus layer of the flounder Platichthys stellatus, which showed antibacterial activity against Staphylococcus epidermidis, Staphylococ-cus aureusand methicillin-resistantS. aureus. | Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus Kosuke Kasai1 2 Takashi Ishikawa1 Takafumi Komata3 Kaori Fukuchi4 Mitsuru Chiba5 Hiroyuki Nozaka1 2 Toshiya Nakamura1 2 Tatsusuke Sato1 2 and Tomisato Miura1 2 1 Division of MedicalLife Sciences Hirosaki University Graduate Schoolof Health Sciences Japan 2 Research Center for BiomedicalSciences Hirosaki University Japan 3 ClinicalLaboratory Shichinohe Hospital Japan 4 ClinicalLaboratory Suzuki Lady s Hospital Kanazawa Japan 5 Graduate Schoolof Comprehensive Human Sciences University of Tsukuba Japan Keywords antibacterial protein methicillin-resistant Staphylococcus aureus MRSA Platichthys stellatus L-amino acid oxidase mucus Correspondence T. Miura Division of MedicalLife Sciences Hirosaki University Graduate Schoolof Health Sciences 66-1 Hon-cho Hirosaki Aomori 036-8564 Japan Fax 81 172 39 5966 Tel 81 172 39 5966 E-mail tomisato@ Received 26 August 2009 revised 26 October 2009 accepted 16 November 2009 doi Fish produce mucus substances as a defensive outer barrier against environmental xenobiotics and predators. Recently we found a bioactive protein in the mucus layer of the flounder Platichthys stellatus which showed antibacterial activity against Staphylococcus epidermidis Staphylococcus aureus and methicillin-resistant S. aureus. In this study we isolated and identified the antibacterial protein from the mucus components of P. stella-tus using a series of column chromatography steps. We then performed gel electrophoresis and cDNA cloning to characterize the protein. The antibacterial protein in the mucus had a molecular mass of approximately 52 kDa with an isoelectric point of and cDNA sequencing showed that it corresponded completely with the peptide sequence of antibacterial protein from the gill. A BLAST search suggested that the

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